南方医科大学学报2016，Vol.36Issue(10)：1357-1363,7.DOI:10.3969/j.issn.1673-4254.2016.10.10
低硒饮食对大鼠肝肾中CD163、CD206、CCR7表达的影响
Effect of low-selenium diet on expressions of CCR7, CD206 and CD163 in the liver and kidney of rats
摘要
Abstract
Objective To investigate the effect of low-selenium diet on the liver and kidneys of rats and explore the role of macrophage polarization into M1 and M2 phenotypes in liver and kidney injuries. Methods Twenty-four rats (12 female and 12 male) were randomly divided into control group and low-selenium group and fed with normal chow (dietary selenium of 0.18 mg/kg) and low-selenium diet (dietary selenium of 0.02 mg/kg) for 109 days. After the feeding, the rats were sacrificed for HE staining to observe liver and kidney pathologies, and immunohistochemistry was performed for analyzing CCR7, CD206, CD163-positive cell numbers in the liver and kidneys. Results The rats in low-selenium group showed severer fibrosis in the liver and kidney than the control group. In either male or female rats in low-selenium group, CCR7 and CD206 expressions in the liver were comparable with those in control group, but CD163 expression was lower than that in the control group (P<0.05 for both female and male rats). In the kidney, the proximal tubule showed a slightly higher while the distal tubule showed a slightly lower CCR7 expression in low selenium group than in the control group (P>0.05). In low-selenium group, a significantly lower CD163 expression in the distal tubule and a significantly higher CD206 expression in the proximal tubule were noted as compared with the control group (P<0.05 in both female and male rats). Compared with the control rats, the male rats in low-selenium group, but not the female rats, showed a significantly lower CD163 expression in the proximal tubule of the kidney (P<0.05);the female but not the male rats in low-selenium group show a higher CD206 expression in the distal tubule (P<0.05). Conclusion Low-selenium diet can cause liver and kidney fibrosis in rats and may inhibit macrophage activation into the M2 phenotype.关键词
硒/肝脏/肾脏/纤维化/巨噬细胞极化Key words
selenium/liver/kidney/fibrosis/macrophage/polarization引用本文复制引用
王丽云,易建华,徐航超,吴晓芳,李丹阳,韩晶..低硒饮食对大鼠肝肾中CD163、CD206、CCR7表达的影响[J].南方医科大学学报,2016,36(10):1357-1363,7.基金项目
国家自然科学基金青年基金（81402639）；陕西省自然科学基金（2015JQ8310）；中央高校基本科研业务费专项资金资助Supported by Natural Science Foundation for the Youth (NSFY) of China (81402639) （NSFY）
