南方医科大学学报2016，Vol.36Issue(10)：1401-1405,5.DOI:10.3969/j.issn.1673-4254.2016.10.18
5-HTs/褪黑素能系统在六味地黄方体外调节黑素代谢中的作用
Role of serotoninergic/melatoninergic system in melanin metabolism in melanocytes exposed to serum of rabbits fed with Liuwei Dihuang decoction
摘要
Abstract
Objective To investigate the effects of Liuwei Dihuang (LWDH) decoction on serotonine (5-HTs), melatonin and the activity of the rate-limiting enzymes ANNAT and HIOMT in cultured human melanocytes and in melanocytes co-cultured with keratinocytes. Methods CCK-8 assay was used to assess the proliferation of melanocytes and melanocytes co-cultured with keratinocytes after treatment with the serum from rabbits fed with LWDH decoction. High-performance liquid chromatography was used to determine 5-HT and melatonin contents, and real-time fluorescent PCR was employed to evaluate the ANNAT and HIOMT activities in the cell cultures. Results The serum from rabbits fed with LWDH Decoction at low doses did not affect the proliferation of melanocytes co-cultured with keratinocytes, but at the concentrations of 20%-40%, the serum significantly inhibited the proliferation of melanocytes, and the effect was optimal with a concentration of 40%(P<0.05). 5-HT and melatonin contents in the cell culture decreased as the serum concentration increased (P<0.05), which was the most obvious with a serum concentration of 40% (P<0.01). Exposure of the cells to low and moderate doses of the serum caused a dose-dependent decrease in AANAT activity (P<0.05), but the serum produced no significant changes in the level of HIOMT mRNA expression in the cells. Couclusion The serotoninergic/melatoninergic system mediate the regulation of melanin metabolism by LWDH Decoction, the mechanism of which may involve 5-HTs, melatonin and ANNAT.关键词
5-羟色胺/褪黑素能系统/黑素细胞/角质形成细胞/共培养/剂量-效应关系/六味地黄方Key words
serotoninergic/melatoninergic system/melanocytes/keratinocytes/co-culture/dose effect/Liuwei Dihuang decoction引用本文复制引用
邓燕,吕琳,杨光,隋育坤..5-HTs/褪黑素能系统在六味地黄方体外调节黑素代谢中的作用[J].南方医科大学学报,2016,36(10):1401-1405,5.基金项目
国家自然科学基金（81202704） Supported by National Natural Science Foundation of China (81202704) （81202704）
