福建农业学报2016，Vol.31Issue(7)：677-682,6.DOI:10.19303/j.issn.1008-0384.2016.07.002
金针菇 JA/SA 信号通路基因鉴定及其对外源 JA/SA 应答
Identification of Genes in JA/SA Signaling Pathways in Flammulina velutipes and Their Response to Exogenous JA/SA
摘要
Abstract
In this study,we identified 4 key genes of JA signaling pathway (COI 1、PDF 1.2、MYC2-1、MYC2-2) and SA signaling pathway (PR1-1、PR1-2、NPR1-1、NPR1-2 )in Flammulina velutipes ,respectively.The results of NBT staining and RT-qPCR demonstrated that JA/SA signaling pathways of F .velutipes responsed to exogenous JA/SA.In addition,the expression levels of these genes of JA/SA signaling pathway significantly increased under the treatment of exogenous 50 μmol · L-1 JA and 500 μmol · L-1 SA for 12 h,respectively.It was worth noting that PDF 1.2 could be considered as the marker gene of JA signaling pathways for its response to exogenous JA obviously. The antagonistic effect between JA and SA signaling pathways may also exist in F.velutipes and the protein NPR1 of SA signaling pathways had inhibitory effect on the gene PDF 1.2 expression level of JA signaling pathways which could be affected by the relative concentration of exogenous JA/SA.关键词
金针菇/茉莉酸/水杨酸/NBT/基因表达Key words
Flammulina velutipes/jasmonic acid/salicylic acid/NBT/gene expression分类
生物科学引用本文复制引用
李肖,汪健芳,严俊杰,陈炳智,谢斌,陈仁良,温志强,江玉姬,谢宝贵..金针菇 JA/SA 信号通路基因鉴定及其对外源 JA/SA 应答[J].福建农业学报,2016,31(7):677-682,6.基金项目
国家重点基础研究发展计划(973项目)(2014CB138302) （973项目）
