福建农业学报2016，Vol.31Issue(7)：690-693,4.DOI:10.19303/j.issn.1008-0384.2016.07.004
惠阳胡须鸡 LPL、FASN 基因多态性的研究
Restriction Fragment Length Polymorphism of LPL and FASN Genes in Huiyang Bearded Chicken
摘要
Abstract
Restriction fragment length polymorphism (RFLP)of LPL and fatty acid synthetase (FASN)genes in Huiyang bearded chickens was obtained to facilitate the selection for dominant genotypes associated with the high-quality meat and fast-growing characteristics in chicken.Genomic DNA was extracted from the chicken blood.The two genes of interest were amplified by PCR.The amplied product of LPL gene was digested by Hinf I,and FASN gene by Hae III,for RFLP analysis.The results showed that (1)in LPL gene,the genotype BB was76.3%,while the two newly discovered genotypes were 1.7% and 22%,with no AA type detected;(2)in FASN gene,genotype BB,CC,and BC were 64.8%,23.4%,and 12.24%,respectively, while the frequencies of B and C genes were0.7041 and 0.2959,respectively.关键词
惠阳胡须鸡/LPL 基因/FASN 基因/限制片段长度多态性Key words
Huiyang bearded chickens/LPL gene/FASN gene/restriction fragment length polymorphism分类
农业科技引用本文复制引用
李红伟,陈圆,鲍淼,田庆庆,贺扬,苏耀辉,邹志冠..惠阳胡须鸡 LPL、FASN 基因多态性的研究[J].福建农业学报,2016,31(7):690-693,4.基金项目
广东省普通高校特色创新项目(自然科学类)(2014KTSCX178) （自然科学类）
惠州学院博士启动基金(156020021) （156020021）
