福建农业学报2016，Vol.31Issue(7)：708-713,6.DOI:10.19303/j.issn.1008-0384.2016.06.008
19份苜蓿种质遗传多样性的 ISSR 分析
Genetic Diversity of Nineteen Alfalfa (Medicago L.)Germplasms Analyzed Using ISSR Markers
摘要
Abstract
Genetic diversities of 1 9 domestic and foreign alfalfa germplasms were analyzed using ISSR markers.A total of 71 effective loci were obtained for the germplasms using 12 ISSR primers, of which 69 loci were polymorphic,i.e.,96.25% of the total.The mean effective number of alleles (Ne ),Nei's gene diversity (H ), Shannon's information index (I ),and polymorphism information content (P IC)were 1.50,0.30,0.46,and 0.33, respectively.The genetic similarity coefficients of the alfalfa germplasms ranged from 0.296 to 0.887,averaging 0.61 7,suggesting ahigh genetic heterogeneityamong the germplasms.According to a cluster analysis,all of the alfalfa germplasms could be divided into 2 groups at the genetic similarity coefficient of 0.522.One group consisted of 1 7 germplasms,accounting for 89.47% of the total.Another group had 2 alfalfa germplasms only.A same result was obtained by the principal component analysis.The information could be used for identification and effective utilization of the alfalfa germplasms.关键词
苜蓿/遗传多样性/ISSR 分析Key words
alfalfa/genetic diversity/ISSR analysis分类
农业科技引用本文复制引用
王健胜,侯桂玲,程立平,谢永凤..19份苜蓿种质遗传多样性的 ISSR 分析[J].福建农业学报,2016,31(7):708-713,6.基金项目
河南科技厅科技攻关项目(KJT142102110171) （KJT142102110171）
平顶山市科技计划项目(2014086) （2014086）
