广东医学2016，Vol.37Issue(18)：2721-2724,4.
川芎嗪抑制人视网膜母细胞瘤WERI－Rb1细胞增殖的机制研究
Effects of tetramethylpyrazine on human retinoblastoma cell line WERI-Rb1 and its mechanism
摘要
Abstract
Objective To characterize the bioactivity of tetramethylpyrazine ( TMP) on human WERI -Rb1 cells and its transcription regulatory mechanism of TMP -mediated down-regulation of CXCR4.Methods (1) WERI-Rb1 cells were treated with TMP and AMD3100.The control cells were treated with DMSO or PBS at the same concentration . The morphology and cell density of WERI -Rb1 cells was observed and recorded by the living cells imager .(2)WERI-Rb1 cells were treated with TMP;then real-time RT-PCR, immunofluorescence and western blot were performed to de-tect the expression levels of CXCR4 and NRF1 in WERI-Rb1 cells treated with TMP.Results (1)TMP and AMD3100 notably inhibited the proliferation of WERI -Rb1 cells, as evidenced by cell morphology and density analysis .(2)TMP down-regulated CXCR4 and NRF1 expression in WERI-Rb1 cells at both mRNA and protein levels (P<0.05), as ev-idenced by real-time RT-PCR, immunofluorescence and western blot assays .Conclusion TMP significantly inhibits the proliferation of WERI -Rb1 cells and down-regulates its expression of CXCR 4 and NRF1.关键词
川芎嗪/WERI-Rb1/趋化因子受体4/核呼吸因子1Key words
tetramethylpyrazine/WERI-Rb1/chemokine receptor 4/nuclear respiratory factor 1引用本文复制引用
徐丽君,吴南丹,杨颖,陈佩,余克明,庄菁..川芎嗪抑制人视网膜母细胞瘤WERI－Rb1细胞增殖的机制研究[J].广东医学,2016,37(18):2721-2724,4.基金项目
国家自然科学基金面上项目 （）
