干旱地区农业研究2016，Vol.34Issue(5)：15-26,12.DOI:10.7606/j.issn.1000-7601.2016.05.03
基于渗透胁迫的马铃薯试管苗抗旱评价体系的构建
Establishment of an evaluation system on drought resistance of potato plantlets based on osmotic stress
摘要
Abstract
To explore the physiological and biochemical mechanism of drought resistance and establish an efficient drought-resistant evaluation system on potato plantlets ,the effect of the PEG (polyethylene glycol 6000) simulated drought stress on the physiological and biochemical parameters for plantlets of potato varieties including CIP 397098 .12 , CIP 391180 .6 ,CIP 391724 .1 ,CIP 392745 .7 ,CIP 392759 .1 ,CIP 393613 .2 ,CIP 397035 .26 ,CIP 302476 .108 , CIP 304345 .102 ,CIP 304405 .47 ,CIP 391930 .1 ,CIP 391931 .1 ,Longshu3 and Atlantic ,and the drought - resistant abilities of testing materials were comprehensively evaluated using the method of Subordinate Function Analysis .The re-sults showed that increase of PEG concentration inhibited the growth of potato seedlings ,especially the length of root sys-tem that was seriously affected .When 9% PEG was applied ,five growth indicators of the samples ,such as plant height , root length ,etc .,were significantly lower than those in the control samples .Therefore ,9% PEG6000 was the optimal concentration to simulate water stress for potato seedlings .According to the value of the comprehensive evaluation , drought resistance of these varieties from strong to weak could be ranked as follows :Longshu3 > CIP 391930 .1 > CIP 397098 .12 > CIP 391931 .1 > CIP 304405 .47 > CIP 392759 .1 > CIP 397035 .26 > CIP 393613 .2 > CIP 302476 .108 >CIP 304345 .102 > CIP 391180 .6 > Atlantic > CIP 391724 .1 > CIP 392745 .7 .关键词
马铃薯/水分胁迫/生理生化指标/隶属函数法Key words
potato/water stress/physiological and biochemical indicators/subordinate function method分类
农业科技引用本文复制引用
娄艳,白江平,杨宏羽,高慧娟,张俊莲,王蒂..基于渗透胁迫的马铃薯试管苗抗旱评价体系的构建[J].干旱地区农业研究,2016,34(5):15-26,12.基金项目
科技部国际科技合作项目（2014DFG31570）；甘肃科技基金（1308RJZA131，1308RJIA005）；兰州科技研究项目 （）
