国际医药卫生导报2016，Vol.22Issue(19)：2909-2912,4.DOI:10.3760/cma.j.issn.1007-1245.2016.19.002
肝细胞癌细胞株HepG2细胞甲胎蛋白基因沉默对Survivin表达的影响
Survivin mRNA affected in HepG2 cells of hepatocellular carcinoma after AFP gene silencing
摘要
Abstract
Objective To study the influence on Survivin expression in HepG2 cells of hepatocellular carcinoma after alpha-fetoprotein (AFP) gene silencing (ie gene is not expressed or is very low-volume expressed).Methods AFP gene expression was down regulated in HepG2 cells by siRNA,and the content of AFP in the supcmatant was detected by ELISA.Survivin mRNA level was tested by RT-PCR.MTT was applied to evaluate cell proliferation.Flow cytometry was applied to evaluate cell apoptosis.Results Compared with previous transfection,the concentration of AFP in the supernatant of observation group gradually reduced after transfection,and at 48 hours after transfection,the degree of decrease in observation group was more obvious.The absorbance of observation group significantly decreased,cell proliferation activity decreased by 45.23％;apoptosis rate of HepG2 cells increased to 36.8％ (by 24.4％),and Survivin mRNA expression decreased by 74.32％.For above cases,there were no significant changes in blank group or control group.Conclusion HepG2 cells of hepatocellular carcinoma after AFP gene silencing can inhibit the mRNA expression of Survivin,so that it reduces the activity of liver cancer cells and increases apoptosis rate of liver cancer cells.关键词
肝细胞癌/HepG2细胞/甲胎蛋白/基因沉默/Survivin表达/mRNAKey words
Hepatocellular carcinoma/HepG2 cells/Alpha-fetoprotein/Gene silencing/Survivin expression/mRNA引用本文复制引用
林旭彪,王百林,梁锐华,张培松..肝细胞癌细胞株HepG2细胞甲胎蛋白基因沉默对Survivin表达的影响[J].国际医药卫生导报,2016,22(19):2909-2912,4.基金项目
Project Supported by Science and Technology Bureau of Jieyang City ([2015]83-49)揭阳市科技局项目(揭市卫[2015]83号-49) （[2015]83-49）
