摘要
Abstract
Objective To investigate the effect of IL-33 on tumor microenvironment and its antitumor effect.Methods 70 mice were randomly divided into control group (10 mice),intervention group (30 mice),model group (30 mice).Intervention group and model group were treated with H22 cells,and IL-33 protein was injected subcutaneously in intervention group after one week.After two weeks,the tumor size of mice,serum levels of IL-2,IL-4,IL-10,IL-12p40,IFN-γ,Thl type immune response in tumor microenvironment,and the expression of OPN and TGF-β in tumor cells were compared.Results After IL-33 intervention,the tumor volume of intervention group was significantly less than that of model group,with statistically significant difference between two groups (P＜0.05).Compared with control group,IL-2,IL-12p40,IFN-γ in model group decreased significantly (P＜0.05),IL-4 increased significantly (P＜0.05);IL-2,IL-12p40,IFN-γ in intervention group increased significantly (P＜0.05),IL-4,IL-10 decreased significantly (P＜0.05),with statistically significant differences between intervention group and model group (P＜0.05).The percentage of CD45+ immune cells in the tumor of intervention group was significantly higher than that of model group,control group (P＜0.05).The percentage of NK and CD8+T immune cells in the tumor of intervention group were significantly higher than those of control group (P＜0.05).The expression of OPN and TGF-β in the tumor tissues in intervention group were lower than those in model group.Conclusions IL-33 can significantly inhibit tumor growth,adjust Th1/ Th2 immune response in tumor microenvironment,and inhibit the expression of OPN and TGF-β protein in tumor cells,which has a potential anti-rumor effect,and provides a new direction for tumor immunotherapy.关键词
IL-33/肿瘤微环境/抗肿瘤Key words
IL-33/Tumor microenvironment/Anti tumor
