甘肃农业科技Issue(9)：13-17,18,6.DOI:10.3969/j.issn.1001-1463.2016.09.005
陈化烟叶产淀粉酶菌株的复筛及产酶条件优化
Screen of Amylase-producing Bacterial Strains of Tabacco and Enzyme Production Conditions Optimize
摘要
Abstract
Based on 224 of amylase-producing bacterial strains isolated from tobacco leaves and their screening for the production of amylase in our previous work,this study aimed to screen amylase-producing bacterial strains which could obviously improve tobacco interior quality by the assessment of sense organ wih smoking,including amylase A-7,A-8,A-9. Different concentrations of the enzyme solution to improve the quality of tobacco leaves shows that 0.29 U/mL of the enzyme solution A-7, 80.67 U/mL of A-8,213.33 U/mL of A-9 on tobacco smoking indicators have improved. The conditon for enzyme production from strain amylase-producing bacterial A-8 is optimized. The result shows that A-8 at pH 6.0,37 ℃conditions,shaking culture 36 hours biomass maximum,60 hours highest activity,the total enzyme activity could be improved by 2.7 times under the optimum conditions.关键词
烟叶/淀粉酶/复筛/产酶条件Key words
Tobacco/Amylase/Screen/Enzyme production conditions分类
农业科技引用本文复制引用
王宝强,吴潇,季秀玲,魏云林,王旺田..陈化烟叶产淀粉酶菌株的复筛及产酶条件优化[J].甘肃农业科技,2016,(9):13-17,18,6.基金项目
云南中烟工业有限责任公司项目（S-6013067）部分内容。 （）
