工业微生物2016，Vol.46Issue(5)：1-10,10.DOI:10.3969/j.issn.1001-6678.2016.05.001
产腈水解酶基因工程菌的产酶诱导条件及培养基优化
Optimization of culture conditions for production of nitrilase by using recombinant E. coli
摘要
Abstract
In this study, the production of nitrilase by using recombinant strain E. coli BL21 (DE3) –pETNYNit was improved by optimization of induced conditions and fermentation medium. The results indicated that the optimal medium were as follows:glucose 0. 2%, glycerol 0. 7% (v/v), peptone 1. 2%, yeast extract 0. 8%, NaCl 0. 3%, (NH4)2SO4 0. 3%, NH4Cl and 0. 13%, Na2HPO4 ·12H2O 1. 04%, KH2PO4 0. 39%, MgSO4 ·7H2O 0. 03%, pH 7. 2; the optimal induced conditions were the following: 0. 5 mmol/L IPTG induced expression after fermented for 4 hours, and then cultured for 14 hours to 16 hours at 28℃ and 240 r/min. After optimization, the nitrilase activity of the recombinant strain increased to (1~0. 9) ×105 U. Compared with that of the wild strain, the enzyme activity increased by more than 50%. At the same time, the culture time of the recombinant strain was about 24 hours, reduced more than 50 hours.关键词
腈水解酶/重组菌/诱导优化/培养基优化Key words
nitrilase/recombinant strain/induction condition optimization/culture medium optimization引用本文复制引用
唐璐敏,薛建萍..产腈水解酶基因工程菌的产酶诱导条件及培养基优化[J].工业微生物,2016,46(5):1-10,10.基金项目
国家高技术研究发展计划(863计划),课题编号SS2014AA022106。 （863计划）
