工业微生物2016，Vol.46Issue(5)：23-30,8.DOI:10.3969/j.issn.1001-6678.2016.05.004
紫色红曲霉Mp-24高产Monacolin K发酵工艺响应面优化
Optimizing fermentation conditions of high-yielding Monacolin K by Monascus purpureus strain Mp-24 using response surface methodology
摘要
Abstract
Response surface methodology and single factor experiment were combined to optimize the submerged culture conditions for the production of Monacolin K using Monascus purpureus strain Mp-24 . Through analyzing the influence of carbon sources, nitrogen sources, carbon content, nitrogen content and culture time, the results showed that optimal fermentation conditions were as follows:lactose( carbon source) , yeast extract( nitrogen source) , carbon content of 7%, nitrogen content of 2% and culture time of 12 d. Monacolin K yield was 167 mg/L. After using Box-Behnken central composite design and response surface analysis, the optimum conditions were: carbon ( lactose) of 8%, nitrogen ( yeast extract) of 3% and culture time of 11 d. Under the optimized conditions, the content of Monacolin K reached 247. 8 mg/L. The optimized fermentation conditions resulted in 1 . 5 times increase of Monacolin K yield in comparison with that of the original conditions.关键词
紫色红曲霉/Monacolin K/发酵工艺/应面优化Key words
Monascus purpureus/Monacolin K/fermentation conditions/response surface methodology引用本文复制引用
Bedelkhan Almagul,任浩,章婷,郑婕施,韩肖飞,蒋冬花..紫色红曲霉Mp-24高产Monacolin K发酵工艺响应面优化[J].工业微生物,2016,46(5):23-30,8.基金项目
国家自然科学基金项目(NO.31270061 （）
NO.31570013)。 （）
