工业微生物2016，Vol.46Issue(5)：31-34,4.DOI:10.3969/j.issn.1001-6678.2016.05.005
离心法分离毕赤酵母活细胞和死细胞
Separation of alive and dead Pichia pastoris cells by centrifugation
摘要
Abstract
A new method of separating alive Pichia pastoris cells from dead Pichia pastoris cells using centrifugation approach was presented, in which alive Pichia pastoris stayed in the up layer of centrifugation solution by changing the density of centrifugation solution through adjusting the ratio of Ficoll-Hypaque solution and 50% polysucrose solution. When the centrifugation method was carried out with the Pichia pastoris cell density at 4. 35×108/mL ~ 1. 13×109/mL (27. 5% of Ficoll-Hypaque solution and 72. 5% of 50% polysucrose solution), it led to 95% and 5% alive stayed in up and down layer of centrifugation solution respectively, which was the highest proportion. And, low ratio of alive Pichia pastoris cells provided higher distribution ratio of up layer centrifugation solution. In summary, this method effectively separate alive Pichia pastoris from dead Pichia pastoris cells.关键词
毕赤酵母/离心/死细胞/分离细胞Key words
Pichia pastoris/centrifugation/dead cells/alive cells引用本文复制引用
刘泰瑜,冯豪,张建国..离心法分离毕赤酵母活细胞和死细胞[J].工业微生物,2016,46(5):31-34,4.基金项目
国家自然科学基金(No.21306112) （No.21306112）
上海市自然科学基金资助项目(No.13ZR1429100) （No.13ZR1429100）
上海高校青年教师培养资助计划(No. slg14037) （No. slg14037）
教育部留学回国人员科研启动基金。 （）
