工业微生物2016，Vol.46Issue(5)：42-49,8.DOI:10.3969/j.issn.1001-6678.2016.05.007
Agrobacterium tumefaciens OAH-01产脲酶发酵培养基优化
Optimization of fermentation culture by urease-producing strain Agrobacterium tumefaciens OAH-01
摘要
Abstract
Urea as the main precursor of ethyl carbamate ( EC ) is a potential carcinogenic component in most of the fermented foods. The methods for elimination of urea must be taken to control the amount of urea. The preferred method for reducing EC content recommended by FDA is adding urease to the wine, because of its advantages such as efficiency, high safety and mild treatment conditions. Microbes are the main source of urease which can be carried out mass production. In this paper, an urease-producing strain Agrobacterium tumefaciens OAH-01 was investigated. Its growth curve and urease-producing curve indicated that the production of enzyme was growth associated model and it could reach the maximum yield in a relatively short time. The effects of fermentation medium composition on urease production by OAH-01 were also investigated. The fermentation medium composition was optimized based on single factor experiment and orthogonal experiment. The optimal medium and conditions were as follows (g/L):peptone 20, yeast extract 5, glucose 5, FeCl3 0. 54, Na2 HPO4 0. 5, KH2 PO4 0. 5, NiSO4 0. 1, pH 7. 0 and cultivation 16 h. Under the optimized conditions, the urease activity of cell suspension was 1. 08 U/mL, it was 2. 4 times than that before optimization. The activity of supernatant after ultrasonic decomposition was 0. 419 U/mL, it was 4. 4 times than that before optimization.关键词
氨基甲酸乙酯/根癌农杆菌/脲酶/发酵优化Key words
ethyl carbamate/Agrobacterium tumefaciens/urease/fermentation optimization引用本文复制引用
侯恩玲,刘良禹,林金雪娇,毛相朝..Agrobacterium tumefaciens OAH-01产脲酶发酵培养基优化[J].工业微生物,2016,46(5):42-49,8.基金项目
山东省科技重大专项(编号:2015ZDZX05003)。 （编号:2015ZDZX05003）
