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产共轭亚油酸菌株的筛选及其等离子体诱变

孙丽慧 王庆山 赵淑艳 修志龙

工业微生物2016,Vol.46Issue(5):50-54,5.
工业微生物2016,Vol.46Issue(5):50-54,5.DOI:10.3969/j.issn.1001-6678.2016.05.008

产共轭亚油酸菌株的筛选及其等离子体诱变

Screening and plasma mutagenesis of conjugated linoleic acid high-producing strain

孙丽慧 1王庆山 1赵淑艳 1修志龙2

作者信息

  • 1. 大连理工大学食品与环境学院,辽宁盘锦124221
  • 2. 大连理工大学生命科学与技术学院,辽宁大连116024
  • 折叠

摘要

Abstract

The strain SC-05 with the ability of synthesize conjugated linoleic acid ( CLA ) was isolated from sauerkraut juice samples. According to gas chromatography analysis, c9, t11-CLA was the only product in the fermentation broth. This strain was identified as Lactobacillus sp. , based on full-length 16S rDNA. The strain SC-05 was induced by low temperature plasma, and the mutant strain A-08 was obtained. The results revealed that the CLA production by the mutant strain A-08 could reach 119. 24 μg/mL, raised 83. 0% compared with that by the original strain. And the mutant strain A-08 also exhibited its good genetic stability.

关键词

共轭亚油酸/筛选/乳杆菌属/等离子体诱变

Key words

conjugated linoleic acid/screening/Lactobacillus/plasma mutagenesis

引用本文复制引用

孙丽慧,王庆山,赵淑艳,修志龙..产共轭亚油酸菌株的筛选及其等离子体诱变[J].工业微生物,2016,46(5):50-54,5.

基金项目

大连理工大学基本科研业务费专项项目(DUT16QY31)。 (DUT16QY31)

工业微生物

1001-6678

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