化工进展2016，Vol.35Issue(10)：3295-3300,6.DOI:10.16085/j.issn.1000-6613.2016.10.039
严格厌氧发酵体系在线尾气监测系统的设计及在梭菌生产生物能源丁醇的应用
The design of online off-gas analysis for anaerobic fermentation and its application in biobutanol production byClostridium as advanced biofuels
摘要
Abstract
Currently,an online off-gas analysis for aerobic or micro-aerobic ethanol fermentation has been established,but the online off-gas analysis forClostridium acetobutylicum and other strict anaerobic fermentation are rarely reported. Therefore,the study on anaerobic fermentation system is based on an online off-gas analysis for aerobic or micro-aerobic ethanol fermentation,and we take a strictly anaerobic fermentation systemofC. acetobutylicum which produces butanol as our research target. Then we get a detection result for theC. acetobutylicum fermentation system by traditional aerobic or micro oxygen gas. Then a design of online off-gas analysis for anaerobic fermentation can be confirmed. Using the online off-gas analysis,the fermentation performance ofC.acetobutylicum under the condition of high cell density was investigated. Compared with the batch fermentation,the fermentation time was shortened,and thus butanol productivity was markedly increased. C.acetobutylicum produced 15.40g/L butanol with a productivity of 0.64g/(L·h) when grown in high cell density. Meanwhile,the butanol production and the productivity were 12.4% and 106%,respectively,higher than those of the control. CO2 and H2 maximum gas rate increased by 60% and 9% respectively,while gas production increased by 20.7% and 41.3%,respectively,while gas data from online off-gas analysis provides an important basis for metabolic analysis of C.acetobutylicum.关键词
尾气在线监测/厌氧发酵/高密度细胞发酵/丁醇/生物燃料Key words
online off-gas analysis/anaerobic fermentation/high cell density fermentation/butanol/biofuel分类
化学化工引用本文复制引用
赵倩倩,杜广庆,陈丽杰,薛闯,白凤武..严格厌氧发酵体系在线尾气监测系统的设计及在梭菌生产生物能源丁醇的应用[J].化工进展,2016,35(10):3295-3300,6.基金项目
国家自然科学基金（21576045,21306020）及辽宁省博士科研启动基金（20141197）项目。 （）
