河南农业科学2016，Vol.45Issue(10)：134-137,4.
河北太行鸡不同群体含黄素单氧化酶3基因多态性检测
Polymorphic Detections of Flavin-containing Monooxygenase 3 in Different Populations of Taihang Chickens in Hebei
摘要
Abstract
In order to study the distribution of T329 S mutation in flavin-containing monooxygenase 3 (FMO3) in different populations of Taihang Chickens in Hebei. Polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP) followed by DNA sequencing assay was used for detecting the frequency of genotype and allele of FMO3 gene in three populations of Taihang Chickens. The results showed that no individual would lay fishy taint eggs in Taihang Chickens of Pingshan,fishy taint suscepti-ble individuals with genotype SS were detected in another two groups. The frequency of T allele was 0. 1298, 0. 0750 and 0. 0842, which was much lower than A allele, respectively. The Chi-square test result showed that there were no significant differences among the three populations with its genotype frequen-cies,and the Chi-square value were 1. 1730(P>0. 05),0. 0351(P>0. 05) and 0. 1885(P>0. 05), which were fit with Hardy-Weinberg equilibrium in different populations of Taihang Chickens in Hebei. As for the genetic parameters showed that the three populations of Taihang Chickens were all intermediate polymorphism on this locus. In conclusion,the frequencies of fishy taint genotype of Taihang Chickens in Hebei was not high and the susceptible individuals could be easily detected by PCR-RFLP method.关键词
河北太行鸡/含黄素单氧化酶3基因/PCR–RFLP/基因型频率Key words
Taihang Chickens in Hebei/FMO3 gene/PCR-RFLP/genotype frequencies分类
农业科技引用本文复制引用
赵思思,胡慧艳,贾青,孙凤莉,锡建中,李晓敏..河北太行鸡不同群体含黄素单氧化酶3基因多态性检测[J].河南农业科学,2016,45(10):134-137,4.基金项目
河北省科技计划项目(14236602D) （14236602D）
