渔业科学进展2016，Vol.37Issue(5)：122-126,5.DOI:10.11758/yykxjz.20150906001
盐度胁迫对三疣梭子蟹（Portunus trituberculatus）鳃中游离氨基酸含量的影响
Effects of Different Salinities on the Free Amino Acids Composition in the Gill of Portunus trituberculatus
摘要
Abstract
In this study, we investigated the composition and function of free amino acids (FAAs) in the gill of Portunus trituberculatus under different salinities. In a 5-day experiment, the contents of FAAs were measured under a series of salinities. We found that in seawater-adapted P. trituberculatus, the concentration of taurine (2.54 mg/g) was the highest, followed by alanine (0.65 mg/g), glutamate (0.64 mg/g), arginine (0.58 mg/g), glycine (0.48 mg/g) and proline (0.25 mg/g). The level of total free amino acids (TOFAA) in the gill increased significantly with the rise in salinity (P<0.05). When salinity was 10, 20, 33, 40, and 50, the concentration of TOFAA in the gill was 5.32 mg/g, 7.26 mg/g, 6.34 mg/g, 7.25 mg/g, and 7.90 mg/g respectively. Non-essential amino acids (NEAA) increased significantly as the salinity was elevated from 10 to 50. Essential amino acids (EAA) was not affected by external salinity change (P>0.05). NEAA includes glutamate (Glu), alanine (Ala), glycine (Gly), aspartic acid (Asp), serine (Ser), cysteine (Cys), tyrosine (Tyr), proline (Pro), and Tauine (Tau), and the rest are EAA. TAA include aspartic acid (Asp), glutamate (Glu), glycine (Gly), and alanine (Ala). The concentrations of free Gly, Glu, Ala, Pro in the gill of P. trituberculatus were raised sharply (P<0.05) with the increase in salinity, whereas the concentration of free Arg decreased (P<0.05). The results suggested that FAAs, especially individual Gly, Glu, Ala, and Arg, could play an important role in salinity adaption. During adaptation, the FAAs pool (mainly NEAA) in the gill may be directly associated to osmoregulation. Change in the FAAs pool in the gill after acclimatization suggested that mainly NEAA might be involved in intracellular osmoregulation. It was also demonstrated that the salinity change mainly affected the NEAA content in the gill.关键词
三疣梭子蟹/鳃/盐度/游离氨基酸(FAAs)/总游离氨基酸(TOFAA)/非必需氨基酸(NEAA)/必需氨基酸(EAA)Key words
Portunus trituberculatus/Gill/Salinity/Free amino acids/TOFAA/NEAA/EAA分类
农业科技引用本文复制引用
付萍,吕建建,刘萍,李健..盐度胁迫对三疣梭子蟹（Portunus trituberculatus）鳃中游离氨基酸含量的影响[J].渔业科学进展,2016,37(5):122-126,5.基金项目
国家自然科学基金面上项目(41576147)、国家自然科学基金青年科学基金项目(41306177)和青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2015ASKJ02)共同资助。 （41576147）
