渔业科学进展2016，Vol.37Issue(5)：127-132,6.DOI:10.11758/yykxjz.20150727002
池塘养殖仿刺参（Apostichopus japonicus）肠含物、附着基和底泥中的菌群结构及其相关性
Correlation Analysis of Flora in the Habitat Environment and in the Gut Contents of Sea Cucumber (Apostichopus japonicus)
摘要
Abstract
Here we studied the properties of flora in the gut content and attachments of sea cucumber (Apostichopus japonicus) as well as in the sediments of pond where sea cucumber were cultured. 16S rDNA PCR denaturing gradient gel electrophoresis (PCR-DGGE) was employed as the major method. The DGGE band numbers were 30.00±1.00, 15.33±1.70, and 21.67±2.62 for gut content, attachments, and pond sediments respectively. This indicated that the gut content had the highest diversity of bacterial species. UPGMA cluster analysis showed that flora from three gut content samples could be categorized into one branch with a similarity value of 0.785, and that flora from attachments and pond sediment samples were clustered into another branch with a similarity value of 0.532. Twenty specific DGGE bands from the three kinds of samples were cut and sequenced. The results showed that Synechococcus sp., Desulfobacterium, Desulfosarcina sp., Polaribacter, Algibacter sp. were the dominant bacterial species in the gut content; Robiginitalea sp., Silicibacter sp. were dominant in attachments; and Proteobacterium, Cytophaga, Desulfosarcina sp. were dominant in the sediment. The specific bacteria in the gut content could be candidates of probiotics in the sea cucumber culture.关键词
PCR-DGGE/仿刺参/肠含物/环境/细菌Key words
PCR-DGGE/Apostichopus japonicus/Gut content/Environment/Bacteria分类
农业科技引用本文复制引用
李彬,廖梅杰,荣小军,张正,王印庚,陈贵平,王岚,范瑞用..池塘养殖仿刺参（Apostichopus japonicus）肠含物、附着基和底泥中的菌群结构及其相关性[J].渔业科学进展,2016,37(5):127-132,6.基金项目
中央级科研院所基本科研业务费专项资金项目(20603022015015)、山东省自主创新成果转化专项(2013ZHZX2A0801)、青岛市海洋经济创新发展区域示范项目(海水健康养殖专用微生态制剂的开发应用与产业化项目)和海洋经济创新发展区域示范重点项目(山东省海水养殖重要疾病高效专用药物研发及产业化)共同资助。 （20603022015015）
