渔业科学进展2016，Vol.37Issue(5)：133-139,7.DOI:10.11758/yykxjz.20150708001
低溶氧胁迫对刺参（Apostichopus japonicus）氧化应激指标的影响
Effects of Hypoxic Stress on Oxidative Stress Indices in Apostichopus japonicus
摘要
Abstract
Apostichopus japonicus that usually habits in the temperate zone, is one of the important aquaculture species at northern China coast. Dissolved oxygen, known as the oxygen content in water, is necessary for the survival of aquatic organisms and directly affects the enzyme activity, metabolism, and growth of aquatic organisms. Therefore, low dissolved oxygen during the hot weather is very harmful for sea cucumber aquaculture and has become an urgent issue. Here we investigated the effects of hypoxic stress on antioxidant capacity of A. japonicus. The experimental group was exposed to hypoxia [(2.0±0.2) mg/L] for 8 h, followed by reoxygenation [(7.0±0.2) mg/L] for 2.5 h. Then tissues from the muscle, respiratory tree, and digestive tract were collected at different time points. Levels of lactic acid, malondialdehyde (MDA), and activities of the antioxidant enzyme system in different tissues and time points were analyzed. It was found that during the exposure to hypoxia, the lactic acid content and activities of total antioxidant capacity (T-AOC), catalase (CAT) and glutathione-S-transferase (GST) were significantly higher compared with the control group (P<0.05), whereas the activity of superoxide dismutase (SOD) became lower (P<0.05) with the extension of hypoxic exposure. The level of MDA in the muscle tissue was significantly lower than in the control group (P<0.05), however, was higher in the respiratory tree and digestive tract. All of the oxidative stress indices gradually returned to normal levels during reoxygenation. These results indicated that as the aerobic metabolism was inhibited during hypoxia, the anaerobic metabolism was increased to maintain the energy supply. The elevated T-AOC, CAT and GST activities and declined SOD activity caused by hypoxia might be an antioxidant strategy to adapt to the hypoxic environment. Our study may provide useful information for the control of hypoxic conditions in sea cucumber aquaculture.关键词
低氧胁迫/刺参/乳酸/丙二醛/抗氧化酶系Key words
Hypoxia stress/Apostichopus japonicus/Lactic acid/Malondialdehyde/Antioxidant enzyme system分类
农业科技引用本文复制引用
李根瑞,任利华,孙国华,杨建敏,韦秀梅,刘兆存,李尚俊,姜汉..低溶氧胁迫对刺参（Apostichopus japonicus）氧化应激指标的影响[J].渔业科学进展,2016,37(5):133-139,7.基金项目
山东省自然科学基金(ZR2013CM029)、山东省现代农业产业技术体系(SDAIT-22-02)、海洋经济创新发展区域示范项目(20130125)、山东省农业重大应用技术创新项目、黄河三角洲学者岗位、山东省良种工程“速生抗病耐高温刺参良种选育”和烟台市科技发展计划项目(2014NC054)共同资助。 （ZR2013CM029）
