生物质化学工程2016，Vol.50Issue(5)：7-11,5.DOI:10.3969/j.issn.1673-5854.2016.05.002
杨木MWL和LCC制备多孔生物载体及其在人体肝细胞培养中的应用
P reparation of P orous Biological Carrier with Aspen MWL and LCC and Application in Culture of Human Hepatocytes
摘要
Abstract
In order to compare the biocompatibility of milled wood lignin ( MWL ) and lignin-carbohydrate complexes ( LCC ) , MWL and LCC were prepared with aspen wood in the present research.Hydrogels were prepared with these materials.Then,porous biological carriers were obtained by freeze-drying. Fourier transform infrared spectrometer ( FT-IR ) and scanning eletron microscope ( SEM ) were applied to elucidate the composition and morphology of the porous biological carriers. Inverted microscope,albumin and glucose kits were used to monitor the morphology and metabolic activity of the cultured hepatocytes.The results indicated that LCC based carriers contained more carbohydrates and less lignin.Therefore,its amphipathicity is better than the MWL based carriers.The porosity of the MWL and LCC based carriers was about 60% with average pore size of 30μm.The results of analysis of cell growth,albumin and glucose metabolism showed that porous biological carriers prepared with aspen LCC could promote the growth of human hepatocytes during culture signficantly. Its biocompatibility is better than that of MWL.关键词
磨木木质素/木质素-碳水化合物复合体/生物载体/肝细胞/生物相容性Key words
milled wood lignin/lignin-carbohydrate complexes/biological carrier/hepatocytes/biocompatibility分类
化学化工引用本文复制引用
吴晨曦,叶哲孜,乐喜,王鹏,谢益民..杨木MWL和LCC制备多孔生物载体及其在人体肝细胞培养中的应用[J].生物质化学工程,2016,50(5):7-11,5.基金项目
国家自然科学基金资助项目(31370574,31300494) （31370574,31300494）
湖北省教育厅科学研究计划资助项目(Q20131402) （Q20131402）
