南华大学学报（自然科学版）2016，Vol.30Issue(3)：19-23,5.
伽马辐照对斑马鱼胚胎谷胱甘肽还原酶活性及基因表达的影响
The Effects of Gamma Irradiation on Activities and Gene Expression of Glutathione Reductase in ZebrafishEmbryos
摘要
Abstract
Glutathione reductase (GR),one of the most important antioxidant enzyme,plays a crucial role in the antioxidant system of an organism. This study was aimed to investigate the effects of gamma irradiation on activities and gene expression of glutathione reductase ( GR) in zebrafish embryos.Different doses of gamma rays (0.01,0.05,0.1,0.5 and 1 Gy) were used to irradiate zebrafish embryos at three developmental stages (gastrula period,segmentation period and pharyngula period),respectively.The activities of GR were assayed and quantitative real-time polymerase chain reaction (qRT-PCR) was performed to measure GRgene expression.The results showed that gamma irradiation modulated the activities and gene expression of GR in a dose-dependent manner.The activities and relative mRNA levelsof GR rose at first and went down later with the increase of the irradiation doses.It was indicated that GR was a suitable bi-omarker for forecasting the gamma irradiation.By upregulating the gene expression of GR,and therefore increasing the synthesis of GR,the zebrafish embryos can enhance the resistance to gamma rays.But such reaction and adjustment are limited.And,the zebrafish embryos at pharyn-gula period had a greater ability to defend the gamma rays than those at other stages.关键词
伽马辐照/斑马鱼胚胎/谷胱甘肽还原酶/基因表达/生物标志物Key words
gamma irradiation/zebrafish embryo/glutathione reductase/gene expression/biomarker分类
医药卫生引用本文复制引用
胡淼,胡南,丁德馨,赵维超,冯永富,李广悦,王永东..伽马辐照对斑马鱼胚胎谷胱甘肽还原酶活性及基因表达的影响[J].南华大学学报（自然科学版）,2016,30(3):19-23,5.基金项目
国防基础科研重点基金资助项目( B3720132001)；国家自然科学基金资助项目( U1401231) （ B3720132001）
