中南医学科学杂志2016，Vol.44Issue(5)：486-489,4.DOI:10.15972/j.cnki.43-1509/r.2016.05.002
胞红蛋白对巨噬细胞氧化损伤的保护作用
Protective effect of CYGB on Macrophage during Oxidative Damage
摘要
Abstract
Objective To observe change of cytoglobin( CYGB) expression and explore the protective role of CYGB during oxidative stress in macrophages. Methods Western blot was used to detect CYGB expression after 0. 5 mmol/L H2O2 treatment for 12 h.Cell model of CYGB overexpression and downexpression was constructed,then to observe the role of CYGB during oxidative stress.After treatment with H2O2 for 12 h,CYGB protein levels,lactate dehydrogenase(LDH) and malondialdehyde( MDA) were detected in each group. Result The expression of CYGB in H2 O2￣treated group was in￣creased compared with control group, the difference was significant ( P<0. 05 ) . The expression of CYGB was higher in 0.5 mmol/L H2O2 treatment+CYGB expression group and lower in 0.5 mmol/L H2O2 treatment+CYGB interference group compared with the 0.5 mmol/L H2O2 treated group (P<0.05).Results showed that LDH,MDA level was decreased in H2O2 treatment+CYGB expression group and increased in H2 O2 treatment+CYGB interference group. Conclusion Expression of CYGB in macrophage was increased during oxidative stress.The CYGB protected against oxidative injury in macrophage.关键词
巨噬细胞/氧化应激/胞红蛋白/过氧化氢Key words
macrophages/oxidative stress/cytoglobin/H2 O2分类
医药卫生引用本文复制引用
屈顺林,朱宇凝,潘文军,李维,王宇菲,谢黎明,姜志胜,范文静..胞红蛋白对巨噬细胞氧化损伤的保护作用[J].中南医学科学杂志,2016,44(5):486-489,4.基金项目
国家自然基金项目(编号:81670424)、国家安全生产重大事故防治关键技术科技项目( hunan￣0013￣2016AQ)、湖南省科技厅项目(编号:2015SK20203,2011TT2051)、湖南自然科学基金项目(编号:2015JJ2118)、湖南省教育厅重点课题(编号:2015JJ2118)、湖南省卫计委科研项目(编号:B2016087)、湖南省研究生科研创新项目( CX2016B480)和中国博士点基金项目(编号:20114324120004)。 （编号:81670424）
