中南医学科学杂志2016，Vol.44Issue(5)：499-503,535,6.DOI:10.15972/j.cnki.43-1509/r.2016.05.005
D￣核糖诱导人肌红蛋白快速非酶糖基化
D-ribose Induced Rapid Non-enzymatic Glycation of Human Myoglobin
摘要
Abstract
Objective To investigate the effect of D￣ribose and D￣glucose on molecular conformation of human myo￣globin( HMb) ,determine the formation of advanced glycation end products ( AGEs) through in vitro glycation,and provide new clues for the prevention and treatment of diabetes mellitus and its complications. Method HMb was expressed in BL21(DE3),purified,and incubated with D￣ribose or D￣glucose.Structural changes of HMb were studied by using UV￣Vis spectroscopy.The formation of AGEs was monitored by Fluorescence spectrum.The molecular weight changes of HMb after reaction were observed by SDS￣PAGE. Results It showed that the heme active site of HMb was altered by interactions with D￣ribose in a time￣dependent manner.Simultaneously,the specific fluorescence of AGEs was found to increase markedly with increasing the incubation time of HMb incubated with D￣ribose.SDS￣PAGE showed that HMb rapidly reacted with D￣ri￣bose and formed higher aggregates.However,D￣glucose has slight effects on the structural change of HMb and the formation of AGEs under the same conditions. Conclusion In summary,this study shows that D￣ribose,but not D￣glucose,rapidly induces ribosylation of human myoglobin and generates AGEs.关键词
核糖/葡萄糖/肌红蛋白/非酶糖基化Key words
D￣ribose/D￣glucose/Myoglobin/non enzymatic glycation分类
医药卫生引用本文复制引用
游咏,刘芳,高淑琴,林英武,格波..D￣核糖诱导人肌红蛋白快速非酶糖基化[J].中南医学科学杂志,2016,44(5):499-503,535,6.基金项目
湖南省教育厅资助科研项目(13C817),湖南省卫生计生委科研计划课题项目( C2016041) （13C817）
