中南医学科学杂志2016，Vol.44Issue(5)：504-509,6.DOI:10.15972/j.cnki.43-1509/r.2016.05.006
蚤休薯蓣皂苷联合顺铂抑制小鼠移植瘤生长及转移
Inhibitory Effects of Rhizome Dioscin Combined with Cisplatin on Subcutaneous Transplanted Tumor Growth and Metastasis of Lewis Lung Carcinoma in Mice
摘要
Abstract
Objective To study the fleas Hugh yam saponins combined cisplatin on Lewis lung carcinoma in mice subcutaneous transplantation tumor growth and metastasis inhibition. Methods 40 C57 BL mice subcutaneously vaccinated Lewis lung cancer cell adenocarcinoma of lung transplantation tumor model is set up, and randomly divided into control group,the RD group,cisplatin group and RD with cisplatin group,since 4 days after inoculation to saline or corresponding drug intervention,29 days after inoculation executed by subcutaneous transplantation tumor tissue expression of CD34 immu￣nohistochemical detection,real￣time fluorescent quantitative PCR detection of subcutaneous transplantation tumor tissue vas￣cular endothelial growth factor ( VEGF) mRNA expression,Western blotting determination of subcutaneous transplantation tumor tissue VEGF,Bcl￣2,Bax protein expression. Results The inhibitory rate and lung surface nodal metastasis inhibi￣tion rate of RD with cisplatin group were higher than the RD and cisplatin group ( P<0.01) ,with Q value of 1.01;RD with cisplatin group improved the index,which was better than the single drug group ( P<0.01) ,but the RD group compared with cisplatin group,had no significant difference (P>0.05). Conclusion Combination of RD and cisplatin has an additive inhibitory effect on subcutaneous transplanted tumor growth and metastasis of Lewis lung carcinoma in mice,which is in￣volved in blockade of angiogenesis and induction of tumor cell apoptosis.关键词
蚤休薯蓣皂苷/顺铂/Lewis肺癌/血管内皮生长因子/凋亡Key words
rhizome dioscin/cisplatin/Lewis lung carcinoma/vascular endothelial growth factor/apoptosis分类
医药卫生引用本文复制引用
邓日强,程江涛,张宏华,杨印楼,刘清毅,谢斌..蚤休薯蓣皂苷联合顺铂抑制小鼠移植瘤生长及转移[J].中南医学科学杂志,2016,44(5):504-509,6.基金项目
广东省韶关市卫生局立项编号 Y15023；韶关市卫计局项目 （）
