摘要
Abstract
Objective To investigate the proinflammatory effect of Treponema pallidum membrane lipoprotein Tp0821 and related signal transduction pathways and provide experimental basis for the clarification of pathogenesis mecha￣nisms of T. pallidum. Methods The recombinant protein Tp0821 ( rTp0821 ) was expressed and the endotoxin in rTp0821 was removed.ELISA was used to detect the expression of proinflammatory cytokines IL￣6,IL￣8,and IL￣1βof human THP￣1 cells stimulated with rTp0821.Furthermore,rTp0821 was used to stimulate human THP￣1 cells pretreated with the ERK1/2 inhibitor PD98059,p38 inhibitor SB203580,and NF￣κB inhibitor BAY11￣7082,respectively,and then ELISA was performed for detection of the expression level of proinflammatory cytokines. Results rTp0821 possesses the ability to in￣duce THP￣1 cells to secret IL￣6,IL￣8,and IL￣1βin dose￣and time￣dependent manners.The expression level of proinflamma￣tory cytokines peaked when THP￣1 cells were stimulated with 1.0μg/mL of rTp0821 for 24 hours.Moreover,when pretreated with PD98059,SP600125,SB203580,and BAY11￣7082,respectively,THP￣1 cells decreases expression of IL￣6 by 63.4%(P<0. 001),4.3% (P>0.05),53.4% (P<0.001),and 73.1% (P<0.0001),IL￣8 by 49.2% (P<0.001)、6.0% (P>0. 05)、41. 1% (P<0.001),and 71.0% (P<0.001),IL￣1β by 36.8% (P<0.01),3.9% (P>0.05),30.3% (P<0.001),and 59.7% ( P<0.001) ,respectively. Conclusion These data indicate that the rTp0821 may activate the THP￣1 cells to pro￣duce the proinflammatory cytokines via ERK1/2,P38,and NF￣κB signal transduction pathways and membrane lipoprotein Tp0821 might be an important pathogenic factor.关键词
梅毒螺旋体/膜脂蛋白/Tp0821/促炎细胞因子/信号转导通路Key words
Treponema pallidum/membrane lipoprotein/Tp0821/proinflammatory cytokines/signal trans￣duction pathways分类
医药卫生
