北方农业学报2016，Vol.44Issue(5)：70-73,4.DOI:10.3969/j.issn.2096-1197.2016.05.16
高效液相色谱法在猕猴桃中维生素C含量分析的应用
Application of Vitamin C content analysis in Actinidia chinensis Planch by HPLC
摘要
Abstract
Improved the HPLC method to measure the Vitamin C in Actinidia chinensis Planch. Using Chromatographic column was Diamonsil C18 (4.6 mm×150 mm,5μm);Detection wavelength of 245 nm;the mobile phase of methyl alcohol∶0.5%phosphoric acid with methanol(5∶95,V/V),as gradient elution;The flow rate was 1.0 mL/min and at 30 ℃temperature. The result showed that Vitamin C concentrations in the scope of 4.72 to 37.76 mg/mL,the linear equation was y=2 780.179 9x+146.4,r=0.999 85.The Vitamin C in Actinidia chinensis Planch determination was 597.23 ±20.55 mg/100g. This method was suited to analyze the Vitamin C in Actinidia chinensis Planch.关键词
猕猴桃/维生素C/高效液相色谱法Key words
Actinidia chinensis planch/Vitamin C/HPLC分类
化学化工引用本文复制引用
郭文川,杨杰,侯茹平,雷凯鹏,杜欢欢,孟怡璠,江海..高效液相色谱法在猕猴桃中维生素C含量分析的应用[J].北方农业学报,2016,44(5):70-73,4.基金项目
陕南秦巴山区生物资源综合开发协同创新中心项目［ QBXT-Z（ P）-15-14］ （）
