山东农业大学学报（自然科学版）2016，Vol.47Issue(5)：647-653,7.DOI:10.3969/j.issn.1000-2324.2016.05.002
内生枯草芽孢杆菌CN181发酵条件优化
Optimization of Fermentation Conditions for Endophytic Bacillus subtilis CN181
摘要
Abstract
To increase the population density of Bacillus subtilis CN181 in the broth, fermentation media and fermentation conditions of B. subtilis CN181 were screened and optimized by single factor and orthogonal tests in this study. The results from single factor experiment showed that the optimum carbon, nitrogen sources and metal ions were corn flour, soybean dregs, K+and Na+. The best medium was composed of corn flour at 15.0 g·L-1, soybean dregs at 10.0 g·L-1, K2HPO4 at 2.0 g·L-1, and NaCl at 2.0 g·L-1 based on the orthogonal test. Finally, the culture conditions were optimized as the following:the initial pH of the fermentation medium at 8.0, the liquid medium volume at 30 mL in a 250 mL flask, the inoculation volume at 2%(v/v), the fermentation temperature at 30℃, the rotation speed at 180 r·min-1 and the fermentation time for 24 h.关键词
枯草芽孢杆菌/内生细菌/发酵条件Key words
Bacillus subtilis/endophytic bacteria/fermentation conditions分类
农业科技引用本文复制引用
康兴娇,贾招闪,申红妙,焦文哲,冉隆贤,甄志先..内生枯草芽孢杆菌CN181发酵条件优化[J].山东农业大学学报（自然科学版）,2016,47(5):647-653,7.基金项目
国家公益性行业(农业)科研专项:果树霜霉病防控技术研究与示范(201203035)。 （农业）
