山东农业大学学报（自然科学版）2016，Vol.47Issue(5)：654-658,5.DOI:10.3969/j.issn.1000-2324.2016.05.003
黄酒中生物胺的HPLC-MS/MS测定
Determination of Biogenic Aminesin Yellow Rice Wine by HPLC-MS/MS
摘要
Abstract
A new HPLC-MS/MS analytical method was developed to detect the biogenic amines (Cadaverine, Histamine, Tyramine, Tryptamine,Putrescine and Phenethylamine ) in Yellow Rice Wine. The analysis was performed using a Atlantis HILIC column (150.0 mm × 2.1 mm, 3 μm) and the mobile phase was consisted of acetonitrile and water (2 mmol/L ammonium acetate, 0.1%formic acid) in the mode of gradient elution program and it was employed in multiple reaction monitoring (MRM) with electrospray ionization (ESI) in the positive mode and the analysis time was fifteen minutes. The detection limits of the six biogenic amines were in the range of 9.7μg/L to 50μg/L. The limit of quantitation was in the range of 28.6 μg/L to 148.6 μg/L. A good linear relationship was observed in the range of 0 μg/L to 1000 μg/L. The average recoveries were in the range of 81.34%to 104.23%for the six biogenic amines. The relative standard deviations (RSD) were between 1.23%and 8.56%. This method is reliable, sensitive and reproducible for the qualitative and quantitative analysis on the biogenic amines in Yellow Rice Wine.关键词
黄酒/生物胺/HPLC-MS/MSKey words
Yellow Rice Wine/biogenic amines/HPLC-MS/MS分类
化学化工引用本文复制引用
许禄,欧杰,王婧,贾素中..黄酒中生物胺的HPLC-MS/MS测定[J].山东农业大学学报（自然科学版）,2016,47(5):654-658,5.基金项目
上海市科委应用技术开发专项资金(2013-116) （2013-116）
(2014-110) （2014-110）
