山东农业大学学报（自然科学版）2016，Vol.47Issue(5)：664-667,4.DOI:10.3969/j.issn.1000-2324.2016.05.005
除草剂筛选小麦转基因植株的有效浓度研究
Study on the Effective Concentration of Herbicide Screening for Transgenic Wheat
摘要
Abstract
The selection marker gene was widely used to detect and identify transgenic plants in biotechnological breeding of crops. For detection of selection marker genes, the molecular techniques such as Polymerase-Chain-Reaction (PCR) and Southern blot were generally applied. However, with the rapid progress of genetic transformation of crop species, a large number of transgenic plants need to be identified timely and usually, bar was designed as the marker gene. The method painting herbicide (glufosinate-ammonium) on leaf has the advantage of quickness and effectiveness for verifying transformation. The results showed that in wheat varieties Lunxuan 987 and Kenong199, the effective selection concentrations of herbicide were 75 mg·L-1 and 125 mg·L-1, respectively. Further, the effective selection concentrations of herbicide were 100 mg·L-1 for detecting the activity of bar protein when Lunxuan 987 was transformed as the donor plant, suggesting this concentration of glufosinate-ammonium might be suitable for large scale detection.关键词
小麦/转基因/除草剂/bar基因/有效浓度Key words
wheat/transgenosis/Herbicide/bar gene/effective concentration分类
生物科学引用本文复制引用
李艺,王悦,王健,赵翔宇,王芳,别晓敏..除草剂筛选小麦转基因植株的有效浓度研究[J].山东农业大学学报（自然科学版）,2016,47(5):664-667,4.基金项目
泰安市大学生科技创新行动计划项目(2014D027) （2014D027）
