山东农业大学学报（自然科学版）2016，Vol.47Issue(5)：674-677,4.DOI:10.3969/j.issn.1000-2324.2016.05.007
近期泰安周边地区鸭病毒性肝炎流行情况调查
The Recent Investigation for the Prevalence of Duck Virus Hepatitis in Peripheral Regions of Tai’an City
摘要
Abstract
In the present study we investigated the prevalence and molecular characterization of Duck hepatitis virus in and around Tai’an area. From the suspected duck population we collected about 10 samples during the period of April to August 2014. Initially these samples were evaluated by necrscopsy, histopathological observation and RT-PCR findings. For further confirmatory studies these samples were subjected to genetic evaluation and homology analysis. The clinical symptoms and histopathological changes revealed that all the samples showed the presence of duck virus hepatitis. Out of the total samples seven were conformed for the presence of viral RNA by RT-PCR. The genetic evaluation and homology analysis of the positive sample on VP0 gene was 98.6%~100%, showed close similarities with previously reported Chinese isolates JS2010 and C-YCW homologies, 98.3%-98.9%. Although these isolates having close phylogenetic relationship but still having some variation in the small branches, which suggested that the present isolates having certain difference from the previous isolates. It is clear that DHV isolate from all the positive samples having less than 76%similarity with DHV-A and DHV-B. Taken together, results suggest that DHV isolated from Shandong province of china is different from the early isolated DHV-A and DHV-B type and is conceder as a new DHV type (DHV-C). Thus for the better control of this economically important disease in Shandong province, it is necessary to use the newly isolated strain for Vaccine preparation.关键词
鸭病毒性肝炎/流行/新型Key words
DVH/prevalence/new type分类
农业科技引用本文复制引用
程子龙,吴海波,于涛,岳瑞超,刘思当..近期泰安周边地区鸭病毒性肝炎流行情况调查[J].山东农业大学学报（自然科学版）,2016,47(5):674-677,4.基金项目
山东省农业科技成果转化资金项目:肉鸭重大疫病综合监控技术集成与示范(20123082) （20123082）
