山东农业科学2016，Vol.48Issue(10)：1-6,6.DOI:10.14083/j.issn.1001-4942.2016.10.001
黑麦草 EST －SSR 分子标记开发及生物信息学分析
Molecular Marker Development and Bioinformatics Analysis of EST -SSR in Ryegrass
摘要
Abstract
To accelerate the application of molecular markers in ryegrass (Lolium perenne L.),the EST -SSR information analysis,molecular marker exploitation and EST function analysis were studied using ryegrass EST data (GenBank /dbEST).Among the 25 752 ryegrass EST sequences,a total of 346 microsatel-lite sequences were found,which amounted for 1.344%.The trinucleotide motifs were the most abundant SSRs (39.60%),followed by the dinucleotide motifs (31.79%).Among the trinucleotide motifs,GGC /CCG had the highest frequency of 8.38%,followed by CGC /GCG (7.51%),GCC /CGG (4.05%)and ATG/TAC (2.02%).Among the dinucleotide motifs,CT/GA had the highest frequency of 14.45%,fol-lowed by GA /CT (10.98%).According to these 346 EST sequences containing SSR,193 EST -SSR primer pairs were designed using Primer Premier 5.0 software.In which,113 (32.66%)primer pairs were found with the score values over 90.The function analysis was performed for the 113 ESTs by the BlastN and BlastX programs in GenBank.Then 72 sequences had homology with 51 kinds of proteins.关键词
黑麦草/EST -SSR/信息分析/引物设计/功能分析Key words
Ryegrass/EST -SSR/Information analysis/Primer design/Function analysis分类
农业科技引用本文复制引用
王秀华,李家丽,蒲艳艳,丛晓翔,林雪婷,于鸿翔,赵岩..黑麦草 EST －SSR 分子标记开发及生物信息学分析[J].山东农业科学,2016,48(10):1-6,6.基金项目
国家大学生创新创业计划项目（201410434051）；山东省高等学校科技计划项目（J09LC15）；山东农业大学青年创新基金项目；山东农业大学博士后基金项目 （）
