山东农业科学2016，Vol.48Issue(10)：7-11,5.DOI:10.14083/j.issn.1001-4942.2016.10.002
BraSDG2 RNA 干扰载体的构建和拟南芥的遗传转化
Construction of BraSDG2 RNAi Vector and Genetic Transformation of Arabidopsis thaliana
摘要
Abstract
Polyploidization is not only a natural phenomenon in plant evolution,but also an important source of plant evolution.The bioinformatics analysis has showed that BraSDG2a (Bra037086)and BraSDG2b (Bra039562)are the homologous genes of AtSDG2.Through the sequence alignment,258 bp DNA conserved fragment of BraSDG2a and BraSDG2b was used to construct the RNAi vector pFGC5941 -BraSDG2 -RNAi. Then the wild type Arabidopsis thaliana (Col -0)was transformed by floral dip method.One transgenic plant was got by PPT screening and PCR detection.The phenotypic observation on T4 generation of transgenic Ara-bidopsis showed that its biological phenotype was similar to Arabidopsis sdg2 mutant.Its plant was dwarf with few seeds.The results laid the foundation for further study on the biological function of BraSDG2.关键词
白菜型油菜/BraSDG2/RNA 干扰/拟南芥/遗传转化Key words
Brassica rapa/BraSDG2/RNA interference/Arabidopsis thaliana/Genetic transformation分类
农业科技引用本文复制引用
周雅智,姜玲,黄勇..BraSDG2 RNA 干扰载体的构建和拟南芥的遗传转化[J].山东农业科学,2016,48(10):7-11,5.基金项目
湖南省高校创新平台开放基金项目 （）
