山东农业科学2016，Vol.48Issue(10)：134-139,144,7.DOI:10.14083/j.issn.1001-4942.2016.10.031
甜樱桃砧木吉塞拉离体茎尖玻璃化法超低温保存研究
Cryopreservation of in vitro Shoot Tips of Sweet Cherry Rootstock Gisela by Vitrification
摘要
Abstract
To maintain the genetic stability and avoid the loss of germplasm resources of sweet cherry ro-otstock Gisela,based on the main factors involved in the process of cryopreservation by vitrification,the or-thogonal experiment was designed to obtain the optimal preservation system for Gisela.The effect of single fac-tor on cryopreservation of Gisela was observed by single factor test.The results showed that after the 90 -day -old tissue culture seedlings were acclimatized at 4 ℃for 3 ~4 weeks,the shoot tips were precultured in preculture solution containing 0.3 mol /L sucrose for 1 day,then permeated in loading solution for 30 minutes, vitrified by PVS3 at 0 ℃ for 60 minutes,stored in liquid nitrogen for 24 hours,rapidly thawed in a water bath at 40 ℃ for 1 minute,washed by unloading solution for twice and each time for 10 minutes,cultured in recov-ering medium and then the shoot tips got the best survival rate and genetic stability.The vitrification cryopr-eservation technique for sweet cherry dwarf rootstock Gisela was built in this study.It provided an efficient way to conserve the germplasm resources of sweet cherry for a long term.关键词
甜樱桃/矮化砧木/吉塞拉/茎尖/超低温冷冻保存/玻璃化法Key words
Sweet cherry/Dwarf rootstock/Gisela/Shoot tip/Cryopreservation/Vitrification分类
农业科技引用本文复制引用
朱东姿,宗晓娟,陈新,王甲威,徐丽,魏海蓉,谭钺,刘庆忠..甜樱桃砧木吉塞拉离体茎尖玻璃化法超低温保存研究[J].山东农业科学,2016,48(10):134-139,144,7.基金项目
“十二五”国家科技支撑计划“主要果树新品种选育”项目“樱桃新品种选育”子课题（2013BAD02B03－3－2）；中俄国际科技合作专项“野果树优异种质资源及种质库技术的引进”（2012DFR30700）；山东省农业良种工程项目“果树种质资源挖掘与种质创新利用研究”［鲁科字（2014）96］；山东省农业科学院重大科技成果培育计划项目“甜樱桃矮化砧木新品种选育及推广利用” （）
