天津科技大学学报2016，Vol.31Issue(5)：1-7,7.DOI:10.13364/j.issn.1672-6510.20160061
生物抗氧化剂电化学检测技术进展
Research Progress in Electrochemical Detection Technology of Biological Antioxidant
摘要
Abstract
Bio-oxidation process and the role of antioxidants(AO)in life sciences are widely recognized as a central issue of modern biology.During the oxidative metabolic process,free radicals and other reactive oxygen species(ROS)are generated and may cause oxidative damages. When excess free radicals or ROS overwhelm AO,destructive and lethal cellular effects occur.Therefore,it is of fundamental importance to understand the role of AO and control the oxidative stress.Different methods have been proposed to study the antioxidant properties of molecules acting through the radical reaction pathway, whereby the AO and the substrate compete for thermally generated peroxyl radicals.These methods using absorbance or fluorescent indicators yield different parameters for evaluating the antioxidant properties.Recently,we have developed the OH radical(·OH)quenching,H2O2 quenching,and H2O2 plus ·OH quenching electrochemcial methods for AO tests,and a different kinetic model was developed to compare the antioxidant activities,which is reviewed in this paper.Using these electrochemical AO detection techniques,ROS generated in situ at the electrode surface can be analyzed,and the redox would be similar to the oxidative metabolic process in vivo.关键词
生物抗氧化剂/活性氧物质/电化学检测/光电化学检测Key words
biological antioxidants/reactive oxygen species/electrochemical detection/photoelectrochemical detection分类
化学化工引用本文复制引用
刘继锋,张强,屠青霞,韩爱玲..生物抗氧化剂电化学检测技术进展[J].天津科技大学学报,2016,31(5):1-7,7.基金项目
国家自然科学基金科学仪器专项资助项目(21127006) （21127006）
