天津科技大学学报2016,Vol.31Issue(5):19-24,6.DOI:10.13364/j.issn.1672-6510.20150146
一株烷烃降解细菌的分离鉴定及其降解特性
Isolation and Identification of an Alkane Degrading Bacterium and its Degradation Characteristics
摘要
Abstract
An alkane degrading bacterium was isolated from the water samples collected from Tianjin sea area.The strain was defined as Citrobacter sp.TUST-S5 according to its physiological and biochemical characteristics as well as 16S rRNA phylogenetic tree.Its degradation rate was determined by GC-MS.Results showed that Citrobacter sp.TUST-S5 had the capacity of degradating C11-C28 alkane and its degradation rate of long chain alkane over C20 was more than 80%,.The growth curve and degradation curve of the strain displayed a change of the degradation rate closely related to the concentra-tion of bacteria.Its emulsifying activity and the hydrophobicity were 47.86%, and 38.30%,.Therefore,Citrobacter sp.TUST-S5 has a good ability to degrade C11-C28 alkane,and will play an important role in remedying marine oil pollution.关键词
烷烃降解细菌/柠檬酸杆菌属/降解率/乳化活性/疏水性Key words
alkane degrading bacteria/Citrobacter/degradation rate/emulsifying activity/hydrophobicity分类
生物科学引用本文复制引用
孙晶,宋东辉,刘凤路,邸富荣..一株烷烃降解细菌的分离鉴定及其降解特性[J].天津科技大学学报,2016,31(5):19-24,6.基金项目
天津市海洋局科技兴海项目(KJXH2013-16) (KJXH2013-16)