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一株烷烃降解细菌的分离鉴定及其降解特性

孙晶 宋东辉 刘凤路 邸富荣

天津科技大学学报2016,Vol.31Issue(5):19-24,6.
天津科技大学学报2016,Vol.31Issue(5):19-24,6.DOI:10.13364/j.issn.1672-6510.20150146

一株烷烃降解细菌的分离鉴定及其降解特性

Isolation and Identification of an Alkane Degrading Bacterium and its Degradation Characteristics

孙晶 1宋东辉 1刘凤路 1邸富荣1

作者信息

  • 1. 天津市海洋资源与化学重点实验室,天津科技大学海洋与环境学院 天津 300457
  • 折叠

摘要

Abstract

An alkane degrading bacterium was isolated from the water samples collected from Tianjin sea area.The strain was defined as Citrobacter sp.TUST-S5 according to its physiological and biochemical characteristics as well as 16S rRNA phylogenetic tree.Its degradation rate was determined by GC-MS.Results showed that Citrobacter sp.TUST-S5 had the capacity of degradating C11-C28 alkane and its degradation rate of long chain alkane over C20 was more than 80%,.The growth curve and degradation curve of the strain displayed a change of the degradation rate closely related to the concentra-tion of bacteria.Its emulsifying activity and the hydrophobicity were 47.86%, and 38.30%,.Therefore,Citrobacter sp.TUST-S5 has a good ability to degrade C11-C28 alkane,and will play an important role in remedying marine oil pollution.

关键词

烷烃降解细菌/柠檬酸杆菌属/降解率/乳化活性/疏水性

Key words

alkane degrading bacteria/Citrobacter/degradation rate/emulsifying activity/hydrophobicity

分类

生物科学

引用本文复制引用

孙晶,宋东辉,刘凤路,邸富荣..一株烷烃降解细菌的分离鉴定及其降解特性[J].天津科技大学学报,2016,31(5):19-24,6.

基金项目

天津市海洋局科技兴海项目(KJXH2013-16) (KJXH2013-16)

天津科技大学学报

OA北大核心CSTPCD

1672-6510

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