天津科技大学学报2016，Vol.31Issue(5)：25-29,68,6.DOI:10.13364/j.issn.1672-6510.20150192
微波辅助双水相萃取黄芪茎总皂苷及其抗氧化活性
Microwave Assisted Aqueous Two-phase Extraction of Total Saponins from Astragalus Stem and the Antioxidant Activity of the Extracts
钟方丽 1王文姣 1王晓林 2曹野1
作者信息
- 1. 吉林化工学院化学与制药工程学院，吉林 132022
- 2. 吉林大学化学学院，长春 130012
- 折叠
摘要
Abstract
Microwave assisted aqueous two-phase extraction was applied to extract the total saponins in Astragalus stems and the antioxidant ability of the extracts in vitro was also investigated.Both single factor and orthogonal tests were em-ployed to optimize the extraction technology.The antioxidant ability of the extraction solution was evaluated according to its scavenging activity and reducing power for superoxide anion free radical.The optimal feed liquid ratio,the microwave power,the ratio between ethanol and water,extraction time and mass concentration of ammonium sulfate were 1∶30(g∶mL),500,W,0.77(mL∶mL),10,min and 0.24,g/mL,respectively.The concentration of the total saponins in Astragalus stems was enriched 2.39,times with aqueous two-phase extraction.The antioxidant activity was gradually enhanced with the increase of mass concentration of extract solution,but it was inferior to that of VC solution.This new technology is stable and the extraction solution has certain antioxidant activity.关键词
黄芪茎/总皂苷/双水相萃取/抗氧化活性Key words
Astragalus stems/total saponins/aqueous two-phase/antioxidant activity分类
医药卫生引用本文复制引用
钟方丽,王文姣,王晓林,曹野..微波辅助双水相萃取黄芪茎总皂苷及其抗氧化活性[J].天津科技大学学报,2016,31(5):25-29,68,6.
