原子能科学技术2016，Vol.50Issue(10)：1748-1755,8.DOI:10.7538/yzk.2016.50.10.1748
柠檬酸对黑麦草修复铀污染土壤的影响
Effect of Citric Acid on Uranium Contaminated Soil Repaired by Lolium perenne
摘要
Abstract
To explore the effects of citric acid concentration on the remediation of urani‐um contaminated soil by Lolium perenne and it’ s physiological characteristics ,pot experiments were conducted to investigate the effects of different citric acid concentra‐tions (0 ,1 ,5 ,10 mmol/kg) on the growth ,physiology and uranium accumulation and distribution of Lolium perenne under the environment of 15 mg/kg uranium stress .The results show that with the increase of citric acid concentrations ,cell inclusion parame‐ters including grow th ,photosynthetic pigments ,soluble protein content ,root vitality , and the activities of glutathione and several enzymes ,in Lolium perenne firstly increase and then decrease .The membranous permeability ,malondialdehyde contents and oxi‐dised glutathione contents gradually increase .The uranium contents in various Lolium perenne positions firstly increase and then decrease ,but the uranium contents in the root are much higher than those in the above‐ground parts .The subcellular uranium dis‐tributions in cells show that the enrichment of uranium is cell w all and residue part>soluble part > organelles and membrane parts ,and the enrichment of uranium firstly increases and then decreases under the same concentration of uranium . The electron microscopy images show that the environment of uranium stress can lead to irreversible damage on Lolium perenne ultrastructures ,while the addition of 5 mmol/kg citric acid can reduce the damage degree .It is concluded that citric acid with low concentrations of 1 and 5 mmol/kg can promote the grow th and remediation efficiency of Lolium perenne in uranium contaminated soil .And 5 mmol/kg citric acid can reach the best effect ,while 10 mmol/kg can limit the grow th of Lolium perenne .关键词
铀胁迫/柠檬酸/生理特性/植物修复Key words
uranium stress/citric acid/physiological characteristic/phytoremediation分类
资源环境引用本文复制引用
杨瑞丽,荣丽杉,杨金辉,刘迎九,谢水波,唐海琼..柠檬酸对黑麦草修复铀污染土壤的影响[J].原子能科学技术,2016,50(10):1748-1755,8.基金项目
国家自然科学基金资助项目（11475080，51408293）；教育部博士点基金资助项目 （）
