针刺研究2016，Vol.41Issue(5)：410-416,7.DOI:10.13702/j.1000-0607.2016.05.009
针刺“百会”透“曲鬓”穴拮抗急性脑出血大鼠炎性损伤的机制研究
Penetrative Needling from“Baihui ”(GV 20)to“Qubin”(GB 7)Antagonizes Inflammatory In-jury in Rats with Cerebral Hemorrhage
摘要
Abstract
Objective To observe the effect of penetrative needling of “Baihui”(GV 20)to “Qubin”(GB 7)on neuro-logic functions and expression of tumor necrosis factor (TNF)-α,interleukin (IL)-6 and toll-like receptor 4 (TLR-4,involving in in-flammatory reactions)in the tissue around the local cerebral hematoma in rats with intracerebral hemorrhage (ICH),so as to pro-vide evidence for clinical treatment of ICH.Methods Fifty-four male Wistar rats were randomly divided into sham control,mo-del and acupuncture groups,and then further divided into three time-point subgroups (1 ,3,7 days after modeling,n = 6/sub-group).The ICH model was established by injection of the rat’s autoblood (50 μL)into the putaman region (P:0.2 mm,R:3.5 mm)in a stereotaxic apparatus and confirmed by Berderson’s neurologic examination grading system (0-3 points).The neuro-logic function was assessed by using Longa’s scoring (5-points)and footfault asymmetry testing [footfault index=(contra faults-ipsi faults)/total steps in 2 min].For penetrative needling,an acupuncture needle was inserted into GV 20 and controlled to ad-vance to GB 7 on the affected side and retained for 30 min,once daily.The expression of TNF-α,IL-6 and TLR-4 in the cerebral tissue around the putaman was detected by immunohistochemistry.Results After penetrative needling stimulation,the increased Longa’s score and footfault asymmetry score in ICH rats were significantly decreased on day 1 ,3 and 7 after modeling (P <0.0 1 ),suggesting an improvement of neurologic function after the treatment.Immunohistochemical staining outcomes of the cerebral tissue surrounding the autoblood injection site showed that the expression levels of TNF-α,IL-6 and TLR-4 proteins on day 1 ,3 and 7 were considerably higher in the model group than in the control group (P <0.0 1 ),and markedly lower in the acupunc-ture group than in the model group (P <0.0 1 ),suggesting a suppression of the proinflammatory factors and TLR-4 levels around the locus of the brain after needling intervention.A positive correlation existed between the expression levels of TLR-4 and IL-6/TNF-α.Conclusion Penetrative needling stimulation of GV 20 to GB 7 can reduce the levels of proinflammatory factors TNF-αand IL-6,and TLR-4 in the ICH tissues in rats with cerebral hemorrhage,which may contribute to its effect in improving neurologi-cal function.关键词
针刺(透刺)/脑出血/肿瘤坏死因子-α/白介素-6/Toll样受体4Key words
Acupuncture (penetrative needling)/Cerebral hemorrhage/Tumor necrosis factor-α/Interleukin-6/Toll-like receptor-4分类
医药卫生引用本文复制引用
陈秋欣,马慧慧,邹伟,孙晓伟,于学平,戴晓红,牛明明,滕伟,包宇,于薇薇..针刺“百会”透“曲鬓”穴拮抗急性脑出血大鼠炎性损伤的机制研究[J].针刺研究,2016,41(5):410-416,7.基金项目
国家自然科学基金(No.81473764、No.81273824) （No.81473764、No.81273824）
黑龙江省自然科学基金重点项目(No.ZD 201204) （No.ZD 201204）
教育部博士点基金项目(No.20102327110003) （No.20102327110003）
黑龙江中医药大学博士创新基金项目(No.2015 bs 03) （No.2015 bs 03）
