针刺研究2016，Vol.41Issue(5)：417-422,446,7.DOI:10.13702/j.1000-0607.2016.05.010
头穴透刺对局灶性脑缺血大鼠海马CA1区酸感受离子通道1a、2b表达的影响
Effect of Scalp-acupuncture Stimulation on Neurological Function and Expression of ASIC 1 a and ASIC 2 b of Hippocampal CA 1 Region in Cerebral Ischemia Rats
摘要
Abstract
Objective To observe the influence of scalp-acupuncture on the expression of acid-sensing ion channels (ASICs)1 a and 2 b of hippocampal CA 1 region in cerebral ischemia (CI)rats,so as to investigate its mechanism underlying im-provement of ischemic stroke.Methods Thirty-two male SD rats were randomly allocated to normal control,model,scalp-acu-puncture and Amiloride group (n =8 in each group).The model of focal CI was established by middle cerebral artery occlusion (MCAO).Scalp acupuncture stimulation was applied to bilateral Dingnieqianxiexian (MS 6)and Dingniehouxiexian (MS 7),once daily for 7 days.Rats of the Amiloride group were fed with Amiloride solution,twice a day for 7 days,and those of the normal control and model groups were grabbled and fixed in the same way with the acupuncture and Amiloride groups.The neurological deficit score was given according to Longa’s method.The expression of hippocampal ASIC 1 a and ASIC 2 b was detected by im-munohistochemistry,and the Ca2+ concentration in the hippocampal tissue assayed using flowing cytometry.Results After the intervention,the neurological deficit score of both the scalp-acupuncture and Amiloride groups were significantly decreased in comparison with pre-treatment (P <0.0 1 ),and the effect of scalp-acupuncture was markedly superior to that of Amiloride in re-ducing neurological deficit score (P <0.05).The expression of ASIC 1 a and ASIC 2 b in the hippocampal CA 1 region and hip-pocampal Ca2+ concentration were significantly up-regulated in the model group compared with the normal control group (P <0.0 1 ),and obviously down-regulated in both scalp-acupuncture and Amiloride groups (P <0.0 1 ,P <0.05),without signifi-cant differences between the two treatment groups in the ASIC 1 a and ASIC 2 b expression and Ca2+ concentration (P >0.05). Conclusion Scalp-acupuncture stimulation can improve neurological function in CI rats,which may be related to its effects in suppressing the increased expression of hippocampal ASIC 1 a and ASIC 2 b proteins and in reducing calcium overload in hip-pocampal neurocytes.关键词
头针/局灶性脑缺血/海马CA1区/酸感受离子通道1a/酸感受离子通道2b/钙离子浓度Key words
Scalp acupuncture/Focal cerebral ischemia/Hippocampal CA1 region/Acid-sensing ion channels (ASIC)1 a/ASIC 2 b/Calcium ion concentration分类
医药卫生引用本文复制引用
田亮,孙润洁,袁博,张星华,张婷卓,李兴兰,王金海,赵敏,鲍英存,商俊芳,鄢琦,张振昶,杜小正,姜华..头穴透刺对局灶性脑缺血大鼠海马CA1区酸感受离子通道1a、2b表达的影响[J].针刺研究,2016,41(5):417-422,446,7.基金项目
甘肃省中医药管理局资助项目(No.GZK-2015-54) （No.GZK-2015-54）
兰州大学第二医院院内中医药课题(No.YJzy2013-39) （No.YJzy2013-39）
2015年甘肃中医药大学研究生创新基金资助项目(No.2016 CX 27) （No.2016 CX 27）
