针刺研究2016，Vol.41Issue(5)：435-439,5.DOI:10.13702/j.1000-0607.2016.05.013
电针结合克罗米芬对多囊卵巢综合征大鼠子宫内膜胰岛素受体和胰岛素受体底物1蛋白表达及胚泡着床的影响
Electroacupuncture Combined with Clomiphene Promotes Pregnancy and Blastocyst Implantation Possibly by Up-regulating Expression of Insulin Receptor and Insulin Receptor Substrate 1 Pro-teins in Endometrium in Rats with PCOS
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA)intervention combined with clomiphene critate (CC)on the blastocyst implantation and pregnancy rate and expression of insulin receptor (INSR)and insulin receptor substrate 1 (IRS 1 )proteins in the endometrium in rats with polycystic ovary syndrome (PCOS),so as to reveal its mechanisms underlying improvement of PCOS.Methods One hundred and twenty-five female SD rats were randomly divided into normal control, PCOS model,medication (CC),EA and EA+CC groups (n =25 in each group,1 5 for checking blastocyst implantation,and 1 0 for Western blot).The PCOS model was established by subcutaneous injection of Dehydroepiandrosterone (DHEA)and fed with high-fat diet.Rats of the normal control group were treated by subcutaneous injection of sesame oil and fed with the normal forage. EA stimulation was applied to “Zhongwan”(CV 1 2),“Guanyuan”(CV 4)and bilateral “Tianshu”(ST 25)for 30 min,3 times a week,5 weeks altogether.Rats of the CC and EA+CC groups were fed with CC (1 00 mg·kg-1 ·d-1 )for 2 days after regular re-striction (30 min,3 times a week,5 weeks altogether).The pregnancy was determined by vaginal smear tests and the number of blastocyst implantation determined by examination of the uterus after execution.The expression of INSR and IRS 1 proteins in the endometrium was detected by Western blot.Results The pregnancy rate and the number of blastocyst implantation were signifi-cantly lower in the model group than in the normal control group (P <0.05),and remarkably increased after EA and EA+CC in-terventions (P <0.05).The effects of EA+CC were obviously superior to those of simple EA and simple medication in raising the pregnancy rate and the number of blastocyst implantation (P <0.05).No significant differences were found between the EA and CC groups in the pregnancy rate and the number of blastocyst implantation (P >0.05).The relative expression levels of both INSR and IRS 1 proteins were markedly lower in the model group than in the normal control group (P <0.05),and significantly higher in both EA and EA+CC groups than in the model group (P <0.05).No significant differences were found between the model and the CC groups and between the EA and EA + CC groups in the expression levels of INSR and IRS 1 proteins (P >0.05).Conclusion EA intervention can improve pregnancy rate and the number of blastocyst implantation in PCOS rats, which may be related to its effects in up-regulating the expression of INSR and IRS 1 proteins in the endometrium.关键词
电针/多囊卵巢综合征/妊娠率/胚泡着床/胰岛素受体/胰岛素受体底物1Key words
Electroacupuncture/Polycystic ovary syndrome/Pregnancy rate/Endometrial implantation of blasto-cyst/Insulin receptor/Insulin receptor substrate 1分类
医药卫生引用本文复制引用
赖毛华,马红霞,宋兴华..电针结合克罗米芬对多囊卵巢综合征大鼠子宫内膜胰岛素受体和胰岛素受体底物1蛋白表达及胚泡着床的影响[J].针刺研究,2016,41(5):435-439,5.基金项目
国家自然科学基金项目(No.81302999) （No.81302999）
