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大连市零售猪肉中沙门氏菌的耐药性检测与分析

杜雄伟 张珂琪 李焱 唐文彦 耿亚娟 王娅君 王佳硕

中国动物检疫2016,Vol.33Issue(10):23-25,30,4.
中国动物检疫2016,Vol.33Issue(10):23-25,30,4.DOI:10.3969/j.issn.1005-944X.2016.10.006

大连市零售猪肉中沙门氏菌的耐药性检测与分析

Antimicrobial Susceptibility Testing ofSalmonella in Retail Pork in Dalian City

杜雄伟 1张珂琪 2李焱 1唐文彦 1耿亚娟 1王娅君 1王佳硕1

作者信息

  • 1. 大连民族大学,辽宁大连 116600
  • 2. 四平市职业技术教育中心,吉林四平 136000
  • 折叠

摘要

Abstract

Objective]To investigate and understand the contamination situation ofSalmonella existed in fresh retail pork in Dalian city,as well as the drug resistance status.[Methods]78 fresh pork samples in supermarkets were collected,thenSalmonellas were isolated and purified by selective medium. After being identified by invA sequence analysis,the drug resistance of theseSalmonella isolates to 12 kinds of antibiotics was detected by the disc agar diffusion method(K-B).[Results]The results showed that 15Salmonella isolates were obtained with the detection rate of 19.23%. The resistance rate to antibiotics was in a range from 0 to 73.3%. 73.3% of the isolates were resistant toGentamycinsulfate,and the resistance rate of other antibiotics decreased in the following order:norlfoxacin hydrochloride(60%)>Florfenicol、Ciprolfoxacin Monohydrochloride(53.3%)>Amoxicillin(13.3%)>Cephalothin、Cefotaxime、Ampicillin(6.7%)>Ceftriaxone,Ceftazidime,Aztreonam,Imipenem(0),altogether 10 drug-resistance profiles were found.[Conclusion]TheSalmonella contamination was severe in the supermarkets and farmer's markets in the development zone of Dalian city,which also showed multi-resistant pattern. The results indicated sufficient attention should be paid attention to the control ofSalmonella.

关键词

零售猪肉/沙门氏菌/耐药性

Key words

retail pork/Salmonella/drug resistance

分类

农业科技

引用本文复制引用

杜雄伟,张珂琪,李焱,唐文彦,耿亚娟,王娅君,王佳硕..大连市零售猪肉中沙门氏菌的耐药性检测与分析[J].中国动物检疫,2016,33(10):23-25,30,4.

基金项目

大连金州新区科技计划项目(KJCX-ZTPY-2014-0013);大连市科技计划项目(2014B11NC094);中央高校自主基金项目(DC2015010020401);“十二五”国家科技支撑计划项目 ()

中国动物检疫

1005-944X

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