中国动物检疫2016，Vol.33Issue(10)：79-85,7.DOI:10.3969/j.issn.1005-944X.2016.10.021
旋毛虫不同发育时期排泄分泌物抗原在免疫学诊断中的应用研究
Application Research forTrichinella spiralis Excretory-secretory Antigens from Different Growth Stages in Immunologic Diagnosis
摘要
Abstract
[Objective]To systematically understand and compare the immunological characters of theTrichinella spiralis excretory-secretory(ES)antigens from different growth stages,so as to explore the serological diagnosis techniques which could be applied to slaughter pigs infected withTrichinella spiralis. [Methods]ES antigens from 10-hour muscle larvae at intestinal stage(10 h ML),30-hour adult at intestinal stage(30 h Ad),3-day adult at adult stage(Ad3),6-day adult and newborn larvae stage(Ad6+NBL),and muscle larvae(ML)ofT.spiralis were used as coating antigen,then the serum of infected pigs in different days within two months was collected and the IgM and IgG antibodies againstTrichinella were detected by ELISA. At last,dynamical change curves of antibodies were drawn and data analysis was made.[Results] 10 h ML and ML ES antigens could be used to detect the anti-Trichinella IgM antibodies from serum of pigs injected by different doses within 35 days after infection,low-dose infection can be detected in about 10 days and the high-dose infection in about 5 days,and Ad3 ES antigens could be applied to detect IgM antibodies when the infection dose was high;ES antigens from Ad3 and ML can be used to detect the anti-Trichinella IgG antibodies from serum of pigs injected by different doses over 35 days after infection,it's worth noting that detection effect by Ad3 ES was better than that of ML ES. [Conclusion]The 10 h ML ES,Ad3 ES and ML ES can be used as coating antigens to detect early infection withTrichinella spiralis,Ad3 ES and ML ES antigens can be used to detectTrichinella spiralis infection in slaughter pigs. This study provided reference and theoretical basis for the sufficient utilization of ES in different stages and for the establishment of a reasonable and effective method towards Trichinella spiralis detection.关键词
旋毛虫/排泄分泌物/猪血清/酶联免疫吸附试验Key words
Trichinella spiralis/excretory-secretory/pig serum/ELISA引用本文复制引用
吴秀萍,孙召金,翟铖铖,刘晓雷,唐斌,刘明远..旋毛虫不同发育时期排泄分泌物抗原在免疫学诊断中的应用研究[J].中国动物检疫,2016,33(10):79-85,7.基金项目
国家自然科学基金 （）
