中国动物检疫2016，Vol.33Issue(10)：86-89,4.DOI:10.3969/j.issn.1005-944X.2016.10.022
猪瘟兔化弱毒间接免疫荧光鉴定方法的建立
Establishment of an Indirect Immunolfuorescence Assay for HCLV Titer Detection
牛成明 1姚静 2杨灵芝 3杨琴 1马景霞 2朱杰1
作者信息
- 1. 山东滨州沃华生物工程有限公司，山东滨州 256606
- 2. 山东省动物病原微生物工程实验室，山东滨州 256606
- 3. 滨州出入境检验检疫局，山东滨州 256603
- 折叠
摘要
Abstract
An indirect immunofluorescence assay(IFA)was developed for detecting HCLV titer rapidly and effectively by using the positive serum of classical swine fever(CSF)as the first antibody and the fluorescein isothiocyanated(FITC)labeled rabbit anti-pig IgG as the second antibody. Results showed the optimum working concentration of thefirst antibody was 1:40,and the dilution ratio of second antibody was 1:32. The IFA displayed clear and specificfluorescence in ST cells inoculated with hog cholera lapinized virus(HCLV)utilizing the above mentioned method,while negative results were obtained in ST cells inoculated with pseudorabies virus(PRV), porcine epidemic diarrhea virus(PEDV)and transmissible gastroenteritis virus(TGEV). Detection for different CSF semi-finished vaccines showed good correlation between Rabbit infection dose(RID)tested with rabbits and 50% tissue culture infective dose(TCID50)tested with IFA,and the fitted curve was RID/mL=2.783TCID50/mL+110107.213.关键词
猪瘟兔化弱毒(HCLV)/间接免疫荧光试验/兔体感染量Key words
hog cholera lapinized virus(HCLV)/indirect immunofluorescence assay/rabbit infection dose分类
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牛成明,姚静,杨灵芝,杨琴,马景霞,朱杰..猪瘟兔化弱毒间接免疫荧光鉴定方法的建立[J].中国动物检疫,2016,33(10):86-89,4.
