中国感染控制杂志2016，Vol.15Issue(10)：764-768,5.DOI:10.3969/j.issn.1671-9638.2016.10.011
阿萨希毛孢子菌和头状地霉菌血流感染的鉴别诊断初探
Differential diagnosis in bloodstream infection with Trichosporon asahii and Geotrichum capitatum
摘要
Abstract
Objective To provide reference for establishing diagnosis and differential diagnosis methods of rare yeast-like fungal bloodstream infection for clinical microbiology laboratory.Methods Trichosporon asahii (T.asa-hii)and Geotrichum capitatum (G.capitatum)bloodstream infection was diagnosed and differentially diagnosed through clinical data analysis,morphological examination,biochemical reactions,and molecular biology technology. Results Two types of yeast-like fungal bloodstream infections in case 1 and case 2 both occurred in leukemia agranulocytosis phase after chemotherapy,such infections were serious and highly similar.The cultivated colonies on blood agar plates of case 1 and case 2 were performed gram stain and microscopic examination.Hyphae,arthro-spores and microconidia were visible in the former,thickness of hyphae branches and length of arthrospores were different,most presented rectangular and barrel shape;the latter can be seen hyphae with transparent septum bro-ken up into arthrospores,presented rectangular shape,did not produce blastoconidia.Identification with API 20C AUX showed that they were T.asahii and G.capitatum.The PCR product sequences were compared with NCBI, suggesting that T.asahii and G.capitatum were at sexual stage.Conclusion Comprehensive application of a varie-ty of technical methods is helpful for improving the diagnosis accuracy of bloodstream infection with yeast-like fungi, identifying Trichosporon and Geotrichum to the species level may help physicians to understand such rare fungal in-fection,choose antifungal agents rationally,and improve clinical prognosis.关键词
血流感染/阿萨希毛孢子菌/头状地霉菌/类酵母样真菌/鉴别诊断Key words
bloodstream infection/Trichosporon asahii/Geotrichum capitatum/yeast-like fungus/differential diagnosis分类
医药卫生引用本文复制引用
钱雪峰,Krupakar Jyothi,赵晔,凌春华,黄建安,季成..阿萨希毛孢子菌和头状地霉菌血流感染的鉴别诊断初探[J].中国感染控制杂志,2016,15(10):764-768,5.基金项目
国家自然科学基金 （）
