中国感染控制杂志2016，Vol.15Issue(10)：780-784,5.DOI:10.3969/j.issn.1671-9638.2016.10.015
2014年北京市西城区40例麻疹患者分离的麻疹病毒基因型及同源性
Genotypes and homology of measles virus isolated from 40 patients with measles in Beijing Xicheng District in 2014
张晶波 1王敬辉 1崔京辉 1于霞丽 2陈萌2
作者信息
- 1. 北京市西城区疾病预防控制中心，北京 100120
- 2. 北京市疾病预防控制中心，北京 100013
- 折叠
摘要
Abstract
Objective To understand the genotypes of measles virus isolated from patients with measles in Beijing Xicheng District in 2014,and timely find out imported measles cases.Methods Measles virus nucleic acid was iden-tified with fluorescent polymerase chain reaction (PCR),then amplified by real-time PCR (RT-PCR),amplified products were sequenced and analyzed.Results 82 specimens from 81 suspected measles patients in Beijing Xicheng District in 2014 were taken,63 were throat swab specimens and 19 were urine specimens.70 cases were positive for measles virus nucleic acid,40 of which were obtained gene sequence through sequencing,37 were positive culture for measles virus.The phylogenetic tree analysis showed that gene sequences of 40 measles virus isolates were be-longed to the same branch as H genotype representative strain Chin9322/H1a and MVi/Hunan.CHN/0.93/7/H1 recommended by World Health Organization.The homology of nucleotide were 98%-98.9% and 96.9%-97.8%respectively,homology of amino acid were 97.3%-99.3% and 95.3%-98% respectively.Conclusion The main genotype of measles cases in Beijing Xicheng District in 2014 was the local genotype (H1 ),surveillance of measles cases should be strengthened further to control and prevent the imported measles cases.关键词
麻疹病毒/核酸检测/H1基因型/流行病学Key words
measles virus/nucleic acid detection/genotype H1/epidemiology分类
医药卫生引用本文复制引用
张晶波,王敬辉,崔京辉,于霞丽,陈萌..2014年北京市西城区40例麻疹患者分离的麻疹病毒基因型及同源性[J].中国感染控制杂志,2016,15(10):780-784,5.
