局解手术学杂志2016，Vol.25Issue(10)：711-714,715,5.DOI:10.11659/jjssx.02E016050
SHBG 基因在人滋养细胞凋亡中的作用
Effect of SHBG gene on the apoptosis of human trophoblastic cells
摘要
Abstract
Objective To investigate the effects of sex hormone-binding globulin (SHBG)gene in the apoptosis of human trophoblastic cells.Methods The siRNA specific-targeting SHBG gene was transfected into human trophoblastic cells and they were divided into six groups:trophoblasts without transfection in normal control groups(group Ⅰ);transfect liposome in blank control groups(group Ⅱ);transfect nonspecific siRNA in negative control groups(group Ⅲ);transfect SHBG siRNA-Ⅰ,SHBG siRNA-Ⅱ,SHBG siRNA-Ⅲ respectively in trans-fection group(group Ⅳ,Ⅴ,Ⅵ).Hoechst 33258 dying method was used to detect cell apoptosis.SHBG and Caspase-3 mRNA profiling and the level of SHBG and caspase-3 protein were detected by real-time PCR and Western blot.Results There was no statistical significant difference in the gene expression and protein level of SHBG and caspase-3 in group Ⅰ,Ⅱ and Ⅲ (P >0.05).In Ⅳ,Ⅴ and Ⅵ group,there was no statistical significant difference in the expression level of SHBG and caspase 3 (P >0.05).Compared with group Ⅰ,Ⅱ and Ⅲ,the a-mount of SHBG gene expression decreased obviously,the caspase-3 mRNA and protein level increased obviously and the trophoblast cell ap-optosis increased markedly (P <0.05).Conclusion Through siRNA interference technology can reduce SHBG gene expression in human trophoblastic cells,and it can lead to excessive apoptosis of human trophoblasts cells.关键词
siRNA干扰/人滋养细胞/SHBG基因/凋亡Key words
siRNA interference/human trophoblastic cells/SHBG gene/apoptosis分类
医药卫生引用本文复制引用
息小雪,练思宇,金镇,孙磊,孙倩,冯冲,王越,张宝..SHBG 基因在人滋养细胞凋亡中的作用[J].局解手术学杂志,2016,25(10):711-714,715,5.基金项目
国家自然科学基金 （）
