局解手术学杂志2016，Vol.25Issue(10)：720-723,4.DOI:10.11659/jjssx.04E016024
鼻咽癌中高表达蛋白激酶及其抑制剂的生物信息学筛选
Bioinformatics screening of up-regulated protein kinases and their inhibitors in nasopharyngeal cancer
摘要
Abstract
Objective To screening up-regulated protein kinases and their inhibitors in order to provide new targets for molecular thera-py of nasopharyngeal cancer.Methods GEO database and SAM software were employed to screen the up-regulated protein kinase gene in nasopharyngeal cancer.Based on DAVID database,the regulating functions of kinases were identified.The inhibitors of up-regulated kinase genes were identified by Selleckchem database.Literature mining was used to screen the potential anti-cancer drugs.Results Totally 2360 differentially expressed genes including 21 up-regulated protein kinases (CHEK1,CHEK2,PRKDC,AURKA,VRK2,STK17A,MELK,NU-AK1,TRPM7,MASTL,AXL,BUB1,BUB1B,CDK4,TTK,CDC7,CASK,AKT3,TBK1 and PBK)were identified in the whole genome profi-ling (Fold Change≥2,P <0.05).The results of function analysis showed the up-regulated genes were enriched in 10 function terms such as‘protein amino acid phosphorylation’‘phosphorylation’‘phosphate metabolic process’‘mitotic cell cycle’‘cell cycle phase’‘regulation of cell cycle’,and so on.The Selleckchem database analysis showed there were 9 up-regulated protein kinases equipped with 51 inhibitors which were proved already.The results of literature mining showed that 18 inhibitors of them had a few studies (less than 10 literatures)in cancer terms,and there was a potential to become new drugs to treat nasopharyngeal cancer.Conclusion A total of 21 up-regulated protein kinases were identified,and they might promote the nasopharyngeal carcinoma by regulating functions such as the cell-cycle control pathway.Their ki-nase inhibitors may have a potential role in anti-cancer treatment,which provided a new target point for molecular therapy of nasopharyngeal cancer.关键词
鼻咽癌/蛋白激酶/激酶抑制剂Key words
nasopharyngeal cancer/protein kinase/kinase inhibitor分类
医药卫生引用本文复制引用
张姝,崔晓波,孙娟..鼻咽癌中高表达蛋白激酶及其抑制剂的生物信息学筛选[J].局解手术学杂志,2016,25(10):720-723,4.基金项目
内蒙古自治区自然科学基金（2014MS0856）；内蒙古医科大学科技百万工程项目 （）
