中国畜牧兽医2016，Vol.43Issue(10)：2509-2517,9.DOI:10.16431/j.cnki.1671-7236.2016.10.001
猪MAP3K5基因CDS序列克隆及生物信息学分析
Cloning and Bioinformatics Analysis of MAP3K5 Gene CDS Sequence in Pig
摘要
Abstract
In this study,we cloned MAP 3K 5 gene cDNA sequence,compared the sequence of MAP3K5 with MAP3K families in pig and other species,and studied their conservative structure domain.Obtained the full-length of MAP 3K 5 gene (5 452 bp)by RACE-PCR,predicted the open reading frame (ORF)and amino acid (AA)sequence,and obtained the information of 31 exons. The low similarity were found in mRNA and amino acid sequences of MAP3K families in pig (<50%).And MAP3K5 had the comparatively high similarity with MAP3K6 in pig.The compar-ative analyzed the mRNA and protein sequence of pig MAP 3K 5 gene with other species MAP 3K 5 gene,founded that the ten species of MAP3K5 mRNA and protein sequence had the high similari-ty (>78%),and pig MAP3K5 had the comparatively high similarity with Bos taurus ,Ovis aries , Canis lupus and Homo sapiens .Function domain analyzed the high similarity protein with MAP3K5 in pig,this research found that pig MAP3K6 protein had the similar conserved function domain with pig MAP3K5.However,the other pig MAP3K families didn’t have the similar con-served function domain with pig MAP3K5.The ten species of MAP3K5 protein had the similar conserved function domain.The results obtained the sequence and molecular structure of MAP 3K 5 gene,and provided an important foundation for further function research of MAP 3K 5 gene in pig.关键词
猪/MAP3K5基因/序列比对/结构域Key words
pig/MAP 3K 5 gene/sequence alignment/function domain分类
生物科学引用本文复制引用
蒲蕾,张跃博,武群清,张龙超,王立贤,岳静伟,张金山,刘欣,王立刚,颜华,侯欣华,高红梅,张恬..猪MAP3K5基因CDS序列克隆及生物信息学分析[J].中国畜牧兽医,2016,43(10):2509-2517,9.基金项目
中国农业科学院科技创新工程(ASTIP-IAS02)；国家"十二五"科技支撑计划项目(2015BAD03B02-2)；国家现代农业产业技术体系(CARS-36) （ASTIP-IAS02）
