中国畜牧兽医2016，Vol.43Issue(10)：2527-2533,7.DOI:10.16431/j.cnki.1671-7236.2016.10.003
基于RNA-Seqs的乌苏里貉agouti基因结构特征预测及分子进化分析
Gene Structure Prediction and Molecular Evolution Analysis of Raccoon Dog agouti Gene based on RNA-Seqs
摘要
Abstract
To in-depth analyze the molecular structure characteristics of agouti gene,using bioin-formatic methods for RNA-Seqs test to get the complete coding sequence of raccoon dog agouti gene,and its coding protein structure was forecasted and analyzed.The results showed that the length of raccoon dog agouti gene cDNA was 530 bp which contained 396 bp open reading frame (ORF),encoded 131 amino acids.Predicting the weight of the raccoon dog agouti protein molecu-lar was 14.41 ku and isoelectric point (pI)was 9.68,as the unstable hydrophohbic protein;It con-tained 1 1 phosphorylation sites,1 glycosylation sites and a long reach 24 amino acid signal pep-tide;Random coil was the main secondary structure.Phylogenetic tree showed that the raccoon dog had a close genetics distance to vuipe and canis familiaris which was consistent with the tradi-tional animal taxonomy.Through the analysis of the molecular structure characteristics of raccoon dog agouti gene,to provide the oretical basis for revealing the influence of the colour diversity molecular genetics mechanism.关键词
貉/agouti基因/agouti信号蛋白/生物信息学Key words
raccoon dog/agouti gene/agouti signaling protein(ASIP)/bioinformatics分类
生物科学引用本文复制引用
张浩,赵家平,吴琼,杨颖,杜站宇,郭跃跃,邢秀梅,徐超,张林波..基于RNA-Seqs的乌苏里貉agouti基因结构特征预测及分子进化分析[J].中国畜牧兽医,2016,43(10):2527-2533,7.基金项目
吉林省科技发展计划(20130206029NY)；中国农业科学院科技创新工程(ASTIP-ISAPS01)；农业部畜禽遗传资源重点实验室开放课题(nzdsys2013-3) （20130206029NY）
